Detailed information of TA system TA060358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 231368..232275
• Replicon: plasmid unnamed
• Accession: NZ_CP014208
• Organism: Pantoea ananatis strain R100

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AW734_RS21965
• Protein ID: WP_022622882.1
• Coordinates: 231808..232275 (+)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8A4JZ23
• Locus tag: AW734_RS21960
• Protein ID: WP_014598335.1
• Coordinates: 231368..231811 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AW734_RS21935	226493..226879	-	387	WP_013027868.1	pyrimidine utilization protein C	-
AW734_RS21940	226905..227657	-	753	WP_019105823.1	pyrimidine utilization protein B	-
AW734_RS21945	227654..228745	-	1092	WP_019105824.1	pyrimidine utilization protein A	-
AW734_RS21950	229144..229833	+	690	WP_019105825.1	HTH-type transcriptional regulator RutR	-
AW734_RS21955	229908..230603	-	696	WP_064374342.1	hypothetical protein	-
AW734_RS22865	230757..230981	-	225	WP_013027863.1	KTSC domain-containing protein	-
AW734_RS21960	231368..231811	+	444	WP_014598335.1	DUF2384 domain-containing protein	Antitoxin
AW734_RS21965	231808..232275	+	468	WP_022622882.1	RES family NAD+ phosphorylase	Toxin
AW734_RS21970	232922..234316	+	1395	WP_014333136.1	flagellar filament capping protein FliD	-
AW734_RS21975	235001..235936	+	936	WP_013027859.1	hypothetical protein	-
AW734_RS21980	235998..236570	-	573	WP_064374343.1	NAD(P)H-dependent oxidoreductase	-
AW734_RS22870	236658..236891	+	234	WP_013027857.1	DUF333 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vipA/tssB / vipB/tssC / vasE/tssK / dotU/tssL / hcp/tssD / clpV/tssH / vgrG/tssI / icmF/tssM / tssF / tssG / fliC / fliC	1..331058	331058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-145)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-89)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17179.64 Da
• Isoelectric Point: 5.1570

>T60358 WP_022622882.1 NZ_CP014208:231808-232275 [Pantoea ananatis]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDFFTLMSIDVPDRLIEWIDIK
QLPDDWAAPEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFSAIVRQAVNIPFLFDARFSRQA

Nucleotide

• Length: 468 bp

>T60358 NZ_CP014208:231808-232275 [Pantoea ananatis]
TTGAAGCTCTATCGCATGACCAAAACGCGCTATCTGGGGTCGGCCTGGAGCGGTTTTGGTGCCCGTGAAGGCGGCGGACG
CTGGAACAGCGTCGGCGTATCGATGGTCTACGCTTCTGAAACTGCTTCCCTCACCATGCTGGAGACGCTTATCCATCTGC
AAAGCGCCAGCGTACTGGACTTTTTTACCCTCATGAGCATTGACGTTCCCGACAGGCTGATTGAATGGATTGATATCAAG
CAACTGCCTGACGACTGGGCCGCACCGGAAGCGCCTGCAGCGCTGCGTTTATTTGGTGATGCCTGGATACAAAGCGGGGG
ATCGGTCGCACTGCGCGTGCCCAGTGCCCTTTCGCCCGTGGAATCGAATTATCTTCTCAATCCGGAGCATCCTGAATTCA
GTGCGATCGTCAGACAGGCGGTAAATATCCCCTTCCTTTTTGATGCACGCTTTTCCCGACAGGCATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.69 Da
• Isoelectric Point: 7.0208

>AT60358 WP_014598335.1 NZ_CP014208:231368-231811 [Pantoea ananatis]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT60358 NZ_CP014208:231368-231811 [Pantoea ananatis]
ATGAAACAGTTCACTTTACCCGTATCCGCTGCAGAACCGGATCGTCTATGGCAATTTGCTGGCCTGAGCAGTGCTGACGG
CGTGATGCTGATGGATCAAATCGATGATGGTCTGGACGGCACGGTAGCCAGTCGCATTGTCACCTGGGCGCAAATCTCTC
AGGCAGAACTGCGCCGTATGACCGGCATTGCGGGCTCAACCTTCACCCGCGGCGTTAAAAGCCGCTTTACTGCGGAGCAG
AGCGAACGGCTGGTGCGTTTTATTCGCGTGATGGATCGTGCGGTTGAGCTGTTTGAGGGCGATAAAGAAAAGGCACGGCA
GTGGCTCAATGAGCCTGCGCGCGCCTTAGGCAATAAAAAACCGGCTGATCTGGTCGCGTCAGAAACCGGGGCTTATGAAG
TGCTGAAACTCATTACCCGGCTTGAACACGGTGTGTATTCTTGA