Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | rlegTA/- |
| Location | 51303..52782 | Replicon | plasmid pR132505 |
| Accession | NC_012854 | ||
| Organism | Rhizobium leguminosarum bv. trifolii WSM1325 | ||
Toxin (Protein)
| Gene name | rlegT | Uniprot ID | C6BAI1 |
| Locus tag | RLEG_RS34315 | Protein ID | WP_012760621.1 |
| Coordinates | 51303..52172 (-) | Length | 290 a.a. |
Antitoxin (Protein)
| Gene name | rlegA | Uniprot ID | - |
| Locus tag | RLEG_RS34320 | Protein ID | WP_012760622.1 |
| Coordinates | 52165..52782 (-) | Length | 206 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RLEG_RS34290 (Rleg_6332) | 48261..48449 | - | 189 | WP_003555203.1 | hypothetical protein | - |
| RLEG_RS34295 (Rleg_6333) | 48645..49358 | - | 714 | WP_012760616.1 | calcium-binding lectin RapA2 | - |
| RLEG_RS38365 (Rleg_6335) | 49812..49964 | + | 153 | WP_012760618.1 | hypothetical protein | - |
| RLEG_RS34300 (Rleg_6336) | 50004..50240 | + | 237 | WP_012760619.1 | 30S ribosomal protein S21 | - |
| RLEG_RS34305 (Rleg_6337) | 50301..50495 | + | 195 | WP_003555197.1 | hypothetical protein | - |
| RLEG_RS34310 (Rleg_6338) | 50603..51082 | + | 480 | WP_012760620.1 | pyridoxamine 5'-phosphate oxidase family protein | - |
| RLEG_RS34315 (Rleg_6339) | 51303..52172 | - | 870 | WP_012760621.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | Toxin |
| RLEG_RS34320 (Rleg_6340) | 52165..52782 | - | 618 | WP_012760622.1 | type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein | Antitoxin |
| RLEG_RS34325 (Rleg_6341) | 53201..54112 | - | 912 | WP_003555190.1 | LysR family transcriptional regulator | - |
| RLEG_RS34330 (Rleg_6342) | 54212..55087 | + | 876 | WP_012760623.1 | aldo/keto reductase family oxidoreductase | - |
| RLEG_RS34335 (Rleg_6343) | 55122..57281 | - | 2160 | WP_012760624.1 | peptidase domain-containing ABC transporter | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..294782 | 294782 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 290 a.a. Molecular weight: 32476.25 Da Isoelectric Point: 5.4176
>T6035 WP_012760621.1 NC_012854:c52172-51303 [Rhizobium leguminosarum bv. trifolii WSM1325]
MAKEIRNVGASVRARLLHLAKASGQSFDLVLTRFALERLLFRLSQSPHANRFVLKGAMLIMSWFDDPHRGTRDLDLLGFG
DPSPDPMLETFLEILAQEADDGVTFDPDTLHVDRIREALDYGGLRLRVIATISGARINLTIDIGFGDALEPGVEILDYPS
MLDFPMPRLRAYARETVIAEKFQAMVMLGRVDSRMKDFYDIWILSRSFDFSDDRLARAIAATFKRRETSIPTNLPDALTD
AFGKDQQKQRQWRAFIEGVAHNPGDLTDVIAEIAAFLMPHAVAAARLDQ
MAKEIRNVGASVRARLLHLAKASGQSFDLVLTRFALERLLFRLSQSPHANRFVLKGAMLIMSWFDDPHRGTRDLDLLGFG
DPSPDPMLETFLEILAQEADDGVTFDPDTLHVDRIREALDYGGLRLRVIATISGARINLTIDIGFGDALEPGVEILDYPS
MLDFPMPRLRAYARETVIAEKFQAMVMLGRVDSRMKDFYDIWILSRSFDFSDDRLARAIAATFKRRETSIPTNLPDALTD
AFGKDQQKQRQWRAFIEGVAHNPGDLTDVIAEIAAFLMPHAVAAARLDQ
Download Length: 870 bp
>T6035 NC_012854:c52172-51303 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGGCTAAGGAAATCAGAAACGTCGGCGCCTCGGTGCGAGCCCGCCTGTTGCACCTCGCCAAGGCAAGCGGGCAAAGCTT
TGATCTGGTCCTGACGCGTTTCGCTCTTGAACGGTTATTGTTCCGGCTCAGTCAGTCGCCCCACGCCAATCGCTTCGTTC
TAAAAGGGGCGATGTTGATAATGAGTTGGTTCGACGACCCGCACCGAGGCACGCGCGACCTCGATCTTTTGGGTTTCGGT
GATCCGAGTCCGGACCCGATGCTTGAGACATTTTTGGAAATTCTGGCGCAAGAGGCCGACGATGGCGTCACGTTCGATCC
CGATACGCTGCATGTGGATCGCATTCGCGAGGCACTGGACTATGGTGGGCTCAGGCTGCGGGTGATCGCTACGATCAGTG
GAGCCCGGATCAACCTGACGATCGACATCGGGTTTGGCGATGCGCTTGAGCCGGGAGTGGAGATCCTGGATTATCCTTCC
ATGCTCGATTTTCCGATGCCGCGGCTCCGGGCCTATGCGCGGGAAACGGTCATTGCCGAGAAGTTTCAGGCCATGGTGAT
GCTGGGGCGAGTAGACAGCCGCATGAAGGATTTCTACGACATTTGGATCCTCAGCAGGTCTTTCGACTTCAGCGATGATC
GGCTGGCGCGTGCAATCGCCGCTACCTTTAAGCGCCGCGAGACATCGATCCCCACCAACCTACCTGACGCCCTAACGGAC
GCCTTTGGCAAGGACCAGCAAAAGCAGCGGCAATGGCGCGCTTTCATTGAAGGCGTTGCACACAATCCTGGCGATCTGAC
AGACGTCATCGCGGAGATTGCCGCATTCCTGATGCCGCACGCGGTTGCAGCTGCGAGGCTGGACCAATGA
ATGGCTAAGGAAATCAGAAACGTCGGCGCCTCGGTGCGAGCCCGCCTGTTGCACCTCGCCAAGGCAAGCGGGCAAAGCTT
TGATCTGGTCCTGACGCGTTTCGCTCTTGAACGGTTATTGTTCCGGCTCAGTCAGTCGCCCCACGCCAATCGCTTCGTTC
TAAAAGGGGCGATGTTGATAATGAGTTGGTTCGACGACCCGCACCGAGGCACGCGCGACCTCGATCTTTTGGGTTTCGGT
GATCCGAGTCCGGACCCGATGCTTGAGACATTTTTGGAAATTCTGGCGCAAGAGGCCGACGATGGCGTCACGTTCGATCC
CGATACGCTGCATGTGGATCGCATTCGCGAGGCACTGGACTATGGTGGGCTCAGGCTGCGGGTGATCGCTACGATCAGTG
GAGCCCGGATCAACCTGACGATCGACATCGGGTTTGGCGATGCGCTTGAGCCGGGAGTGGAGATCCTGGATTATCCTTCC
ATGCTCGATTTTCCGATGCCGCGGCTCCGGGCCTATGCGCGGGAAACGGTCATTGCCGAGAAGTTTCAGGCCATGGTGAT
GCTGGGGCGAGTAGACAGCCGCATGAAGGATTTCTACGACATTTGGATCCTCAGCAGGTCTTTCGACTTCAGCGATGATC
GGCTGGCGCGTGCAATCGCCGCTACCTTTAAGCGCCGCGAGACATCGATCCCCACCAACCTACCTGACGCCCTAACGGAC
GCCTTTGGCAAGGACCAGCAAAAGCAGCGGCAATGGCGCGCTTTCATTGAAGGCGTTGCACACAATCCTGGCGATCTGAC
AGACGTCATCGCGGAGATTGCCGCATTCCTGATGCCGCACGCGGTTGCAGCTGCGAGGCTGGACCAATGA
Antitoxin
Download Length: 206 a.a. Molecular weight: 22554.03 Da Isoelectric Point: 10.1682
>AT6035 WP_012760622.1 NC_012854:c52782-52165 [Rhizobium leguminosarum bv. trifolii WSM1325]
MPSSDTQRQIARTVLTERGIARLRELRNAGVTGATMSRMERDGEVLRLARGLYQLPDAELDVNHSMAEVAKRAPKAVICL
VSALAFHGLTDQLPRQIWLAIGRKDWAPKPDGPAMRIVRFTESLLNESVETHVIEGVPVKVFGIAKTIADCFRYRNKIGL
SIAMEGLQEALRQRRTSPGEIANQAERGGVGSVIRPYLEALTANG
MPSSDTQRQIARTVLTERGIARLRELRNAGVTGATMSRMERDGEVLRLARGLYQLPDAELDVNHSMAEVAKRAPKAVICL
VSALAFHGLTDQLPRQIWLAIGRKDWAPKPDGPAMRIVRFTESLLNESVETHVIEGVPVKVFGIAKTIADCFRYRNKIGL
SIAMEGLQEALRQRRTSPGEIANQAERGGVGSVIRPYLEALTANG
Download Length: 618 bp
>AT6035 NC_012854:c52782-52165 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGCCCAGTTCCGACACTCAACGCCAAATCGCCCGGACCGTGCTAACGGAGCGCGGAATAGCACGTTTGAGGGAACTGCG
TAATGCAGGTGTGACAGGGGCCACAATGAGCCGAATGGAACGGGATGGCGAGGTGCTGCGGCTTGCCCGCGGTCTCTACC
AACTTCCTGATGCGGAGCTTGATGTCAACCACAGTATGGCAGAGGTTGCCAAACGCGCGCCGAAAGCCGTTATCTGCCTC
GTTTCGGCCTTGGCATTCCACGGCCTGACGGATCAGCTTCCGAGACAAATCTGGCTTGCCATAGGCCGTAAGGACTGGGC
CCCGAAACCGGACGGTCCCGCTATGCGCATCGTACGTTTCACCGAAAGCCTTCTCAACGAAAGCGTCGAAACCCATGTCA
TCGAAGGCGTTCCCGTGAAGGTCTTTGGAATAGCCAAGACAATCGCCGATTGCTTTCGCTATCGCAACAAGATCGGCCTT
TCGATAGCGATGGAGGGGCTGCAGGAGGCACTGCGGCAACGAAGGACTAGTCCTGGCGAAATCGCCAACCAGGCCGAACG
CGGTGGCGTCGGATCCGTGATCCGGCCGTACCTCGAGGCGCTGACCGCCAATGGCTAA
ATGCCCAGTTCCGACACTCAACGCCAAATCGCCCGGACCGTGCTAACGGAGCGCGGAATAGCACGTTTGAGGGAACTGCG
TAATGCAGGTGTGACAGGGGCCACAATGAGCCGAATGGAACGGGATGGCGAGGTGCTGCGGCTTGCCCGCGGTCTCTACC
AACTTCCTGATGCGGAGCTTGATGTCAACCACAGTATGGCAGAGGTTGCCAAACGCGCGCCGAAAGCCGTTATCTGCCTC
GTTTCGGCCTTGGCATTCCACGGCCTGACGGATCAGCTTCCGAGACAAATCTGGCTTGCCATAGGCCGTAAGGACTGGGC
CCCGAAACCGGACGGTCCCGCTATGCGCATCGTACGTTTCACCGAAAGCCTTCTCAACGAAAGCGTCGAAACCCATGTCA
TCGAAGGCGTTCCCGTGAAGGTCTTTGGAATAGCCAAGACAATCGCCGATTGCTTTCGCTATCGCAACAAGATCGGCCTT
TCGATAGCGATGGAGGGGCTGCAGGAGGCACTGCGGCAACGAAGGACTAGTCCTGGCGAAATCGCCAACCAGGCCGAACG
CGGTGGCGTCGGATCCGTGATCCGGCCGTACCTCGAGGCGCTGACCGCCAATGGCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Hila Sberro et al. (2013) Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning. Molecular Cell 50(1):136-48. [PubMed:23478446]