Detailed information of TA system TA060344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 5445064..5445703
• Replicon: chromosome
• Accession: NZ_CP014205
• Organism: Pseudomonas glycinae strain MS586

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3T0K2L6
• Locus tag: AWU82_RS24910
• Protein ID: WP_064384144.1
• Coordinates: 5445064..5445246 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AWU82_RS24915
• Protein ID: WP_064382776.1
• Coordinates: 5445278..5445703 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWU82_RS24870	5440129..5440845	+	717	WP_064382772.1	AzlC family ABC transporter permease	-
AWU82_RS24875	5440824..5441141	+	318	WP_007952614.1	AzlD domain-containing protein	-
AWU82_RS24880	5441058..5441984	-	927	WP_011331998.1	LysR family transcriptional regulator	-
AWU82_RS24885	5442354..5442509	+	156	WP_170928946.1	hypothetical protein	-
AWU82_RS24890	5442678..5442896	+	219	WP_007952610.1	4-oxalocrotonate tautomerase family protein	-
AWU82_RS24895	5442893..5443537	+	645	WP_064382773.1	DsbA family oxidoreductase	-
AWU82_RS24900	5443571..5444281	+	711	WP_064382774.1	SDR family oxidoreductase	-
AWU82_RS24905	5444340..5444606	-	267	WP_064382775.1	DUF3077 domain-containing protein	-
AWU82_RS24910	5445064..5445246	+	183	WP_064384144.1	type II toxin-antitoxin system HicA family toxin	Toxin
AWU82_RS24915	5445278..5445703	+	426	WP_064382776.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6810.86 Da
• Isoelectric Point: 10.8173

>T60344 WP_064384144.1 NZ_CP014205:5445064-5445246 [Pseudomonas glycinae]
MKYSEFRRWLKAQGVEFQTGKGSHFKVSLNGRSTVFPDHGAKEMGEGLRKSIIKQLGLKD

Nucleotide

• Length: 183 bp

>T60344 NZ_CP014205:5445064-5445246 [Pseudomonas glycinae]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGTTGAGTTTCAAACCGGCAAGGGCAGCCATTTCAAGGT
CTCCTTGAATGGCAGATCAACTGTCTTTCCCGACCACGGAGCCAAGGAGATGGGCGAAGGGTTGAGAAAATCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15544.63 Da
• Isoelectric Point: 4.3686

>AT60344 WP_064382776.1 NZ_CP014205:5445278-5445703 [Pseudomonas glycinae]
MFEYALEVHEEPSSVWLSCAEIPEMHAAGDTLEEALDGAIDAMETALSIYVDDRRLIPTGDVGEQESDAVLRLPALTAAK
VGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFLHHSKIENVERALQQLGRRILLSVEAA

Nucleotide

• Length: 426 bp

>AT60344 NZ_CP014205:5445278-5445703 [Pseudomonas glycinae]
ATGTTCGAATATGCGTTGGAAGTCCACGAGGAGCCGAGCAGTGTCTGGTTGTCCTGCGCAGAAATTCCAGAGATGCACGC
CGCTGGCGACACCCTCGAAGAAGCGCTCGACGGCGCTATTGATGCTATGGAAACGGCATTGTCGATCTACGTTGATGATC
GCCGGTTGATTCCGACAGGTGATGTGGGAGAGCAGGAAAGTGACGCGGTGTTGCGTTTGCCTGCACTGACTGCTGCGAAG
GTTGGACTCTGGAATACGCTTTTGGAGTCTGGAGTCAGCAAGGCTGAACTTGCACGTCGGTTGGGGGTGCAACGACCCCA
GGTAGATCGGTTAGTCGATTTCTTGCATCACTCCAAGATTGAGAATGTCGAGCGTGCATTGCAGCAGCTTGGGCGCCGGA
TTTTGCTTTCGGTTGAGGCTGCGTAG

Structures

Toxin

• AlphaFold DB: A0A3T0K2L6