Detailed information of TA system TA060343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 5296327..5296843
• Replicon: chromosome
• Accession: NZ_CP014205
• Organism: Pseudomonas glycinae strain MS586

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AWU82_RS24220
• Protein ID: WP_064382676.1
• Coordinates: 5296562..5296843 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0C1VYZ2
• Locus tag: AWU82_RS24215
• Protein ID: WP_039773014.1
• Coordinates: 5296327..5296572 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWU82_RS24180	5291598..5292203	+	606	WP_064382671.1	adenylyl-sulfate kinase	-
AWU82_RS24185	5292200..5292985	+	786	WP_064382672.1	aspartyl/asparaginyl beta-hydroxylase domain-containing protein	-
AWU82_RS24190	5292973..5293947	+	975	WP_064382673.1	aspartyl beta-hydroxylase	-
AWU82_RS24195	5293913..5294671	-	759	WP_007957673.1	slipin family protein	-
AWU82_RS24200	5294674..5295201	-	528	WP_064382674.1	serine protease	-
AWU82_RS24205	5295398..5295859	+	462	WP_064382675.1	YbaK/EbsC family protein	-
AWU82_RS24210	5295989..5296231	+	243	WP_039773016.1	DUF2789 domain-containing protein	-
AWU82_RS24215	5296327..5296572	+	246	WP_039773014.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AWU82_RS24220	5296562..5296843	+	282	WP_064382676.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AWU82_RS24225	5296968..5297396	+	429	WP_011331882.1	winged helix DNA-binding protein	-
AWU82_RS24230	5297393..5299456	+	2064	WP_064382677.1	FUSC family protein	-
AWU82_RS24235	5299453..5299662	+	210	WP_007957657.1	DUF1656 domain-containing protein	-
AWU82_RS24240	5299659..5300546	+	888	WP_064382678.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10985.87 Da
• Isoelectric Point: 10.5375

>T60343 WP_064382676.1 NZ_CP014205:5296562-5296843 [Pseudomonas glycinae]
MTYKLEFLPSAHKEWNKLGHTLRDQFKKKLAERLERPRIPADSLHGMPDCYKIKLKASGYRLVYQVIEERVVVSVVAVGK
RERSDVYEKAKRR

Nucleotide

• Length: 282 bp

>T60343 NZ_CP014205:5296562-5296843 [Pseudomonas glycinae]
ATGACCTATAAGCTCGAATTTCTGCCTTCCGCTCACAAAGAGTGGAACAAACTGGGTCATACGCTGCGTGATCAATTTAA
AAAGAAGCTGGCTGAGCGTCTTGAGCGTCCGCGTATTCCGGCTGACTCGCTCCACGGCATGCCTGATTGCTACAAGATCA
AACTCAAAGCGTCCGGTTATCGACTGGTTTACCAAGTGATCGAAGAGCGGGTGGTTGTCTCGGTAGTCGCCGTGGGTAAG
CGCGAGCGAAGCGACGTCTACGAAAAAGCGAAAAGACGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8776.06 Da
• Isoelectric Point: 4.4988

>AT60343 WP_039773014.1 NZ_CP014205:5296327-5296572 [Pseudomonas glycinae]
MQSVLADMAVSVSELKKNPSAVLNGAHGGAVAVLNHNRVMGYMVPADVYEAMMERLDDLELAEIVRSRSHETPIPVSLDD
L

Nucleotide

• Length: 246 bp

>AT60343 NZ_CP014205:5296327-5296572 [Pseudomonas glycinae]
ATGCAAAGTGTTCTGGCCGATATGGCTGTCAGTGTTTCTGAACTGAAGAAAAACCCCTCCGCAGTGCTAAACGGCGCGCA
CGGAGGAGCAGTTGCTGTCCTGAATCACAACCGGGTCATGGGCTACATGGTGCCGGCTGACGTTTACGAAGCCATGATGG
AGCGGCTCGATGATCTCGAGTTGGCAGAAATCGTTCGTTCTCGCAGTCACGAGACTCCCATACCGGTAAGCCTGGATGAC
CTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0C1VYZ2