Detailed information of TA system TA060340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1740299..1740950
• Replicon: chromosome
• Accession: NZ_CP014201
• Organism: Lacticaseibacillus rhamnosus strain BFE5264

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: AWJ15_RS08750
• Protein ID: WP_005686631.1
• Coordinates: 1740299..1740682 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: AWJ15_RS08755
• Protein ID: WP_005686632.1
• Coordinates: 1740702..1740950 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWJ15_RS08735	1739335..1739862	-	528	WP_005686630.1	QueT transporter family protein	-
AWJ15_RS08740	1739900..1740097	-	198	WP_064536013.1	hypothetical protein	-
AWJ15_RS08750	1740299..1740682	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWJ15_RS08755	1740702..1740950	-	249	WP_005686632.1	antitoxin	Antitoxin
AWJ15_RS08760	1741062..1742201	-	1140	WP_005686633.1	alanine racemase	-
AWJ15_RS08765	1742188..1742562	-	375	WP_005686634.1	holo-ACP synthase	-
AWJ15_RS08770	1742731..1744239	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
AWJ15_RS08775	1744513..1745901	-	1389	WP_005686636.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ClpL	-	1711671..1895542	183871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T60340 WP_005686631.1 NZ_CP014201:c1740682-1740299 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T60340 NZ_CP014201:c1740682-1740299 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAATAACGTTGGCAATCACTATAGTCCCACCGTCATTGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACTCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
AGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT60340 WP_005686632.1 NZ_CP014201:c1740950-1740702 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT60340 NZ_CP014201:c1740950-1740702 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGTGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAGTTGATTT
CGGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19