60337

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4638815..4639359 Replicon chromosome
Accession NZ_CP014168
Organism Sphingomonas panacis strain DCY99

Toxin (Protein)


Gene name mazF Uniprot ID A0A1B3ZF87
Locus tag J0A91_RS21495 Protein ID WP_069206609.1
Coordinates 4638815..4639138 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1B3ZF89
Locus tag J0A91_RS21500 Protein ID WP_069206610.1
Coordinates 4639135..4639359 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0A91_RS21470 4634950..4635369 - 420 WP_069207553.1 hypothetical protein -
J0A91_RS21475 4635629..4636213 + 585 WP_069207554.1 aminodeoxychorismate/anthranilate synthase component II -
J0A91_RS21480 4636210..4637205 + 996 WP_069206606.1 anthranilate phosphoribosyltransferase -
J0A91_RS21485 4637202..4637987 + 786 WP_069206607.1 indole-3-glycerol phosphate synthase TrpC -
J0A91_RS21490 4638219..4638695 + 477 WP_069206608.1 cyclic pyranopterin monophosphate synthase MoaC -
J0A91_RS21495 4638815..4639138 - 324 WP_069206609.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J0A91_RS21500 4639135..4639359 - 225 WP_069206610.1 antitoxin MazE family protein Antitoxin
J0A91_RS21505 4639493..4640761 - 1269 WP_150127023.1 hypothetical protein -
J0A91_RS21510 4641031..4642215 + 1185 WP_069206612.1 molybdopterin molybdotransferase MoeA -
J0A91_RS21515 4642284..4642961 + 678 WP_069206613.1 transcriptional repressor LexA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4631592..4640761 9169


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(6-69)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11321.30 Da        Isoelectric Point: 10.5887

>T60337 WP_069206609.1 NZ_CP014168:c4639138-4638815 [Sphingomonas panacis]
VKRGDLVTIALSGDFGKPRPALIIQSDQFAETGTVTVLLVSRTLVDAPLIRTTIEPTPANGLRARSQIMVDKAMSVKRDK
IGATIGSLDAEAMLAVTRALAVFFAIA

Download         Length: 324 bp

>T60337 NZ_CP014168:c4639138-4638815 [Sphingomonas panacis]
GTGAAGCGGGGCGATCTCGTCACCATTGCGCTGTCGGGGGATTTCGGCAAACCGCGCCCGGCGCTCATCATCCAGTCCGA
TCAGTTCGCCGAAACCGGCACGGTGACGGTGTTGCTCGTCTCCAGAACGTTGGTGGACGCGCCGCTGATCCGCACGACGA
TCGAGCCGACGCCTGCCAATGGCCTGCGGGCGCGTTCGCAGATCATGGTGGACAAGGCGATGTCGGTGAAGCGCGATAAG
ATCGGCGCGACGATCGGCAGCCTCGATGCCGAGGCGATGCTGGCGGTGACGCGTGCGCTGGCGGTGTTCTTCGCGATTGC
GTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8057.14 Da        Isoelectric Point: 5.0633

>AT60337 WP_069206610.1 NZ_CP014168:c4639359-4639135 [Sphingomonas panacis]
MPAPLNERVRKRRDALRAAGLRPIQIWVPDTRRAGFAEECRRQARIVAAADAADADLDAFLDAALADMDPAGEA

Download         Length: 225 bp

>AT60337 NZ_CP014168:c4639359-4639135 [Sphingomonas panacis]
ATGCCAGCACCTCTCAATGAACGGGTCCGCAAGCGCCGCGACGCACTGCGTGCCGCTGGTTTGCGCCCGATTCAGATTTG
GGTGCCCGACACGCGACGCGCCGGCTTCGCCGAGGAATGCCGGCGGCAGGCACGCATCGTCGCGGCGGCCGATGCCGCCG
ACGCCGATCTGGACGCATTCCTAGACGCCGCGCTTGCCGATATGGACCCCGCAGGCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3ZF87

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3ZF89

References