Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | sanaTA/- |
| Location | 21935..23283 | Replicon | plasmid 1 |
| Accession | NC_008573 | ||
| Organism | Shewanella sp. ANA-3 | ||
Toxin (Protein)
| Gene name | sanaT | Uniprot ID | A0A5B8R339 |
| Locus tag | SHEWANA3_RS21760 | Protein ID | WP_011711498.1 |
| Coordinates | 22342..23283 (+) | Length | 314 a.a. |
Antitoxin (Protein)
| Gene name | sanaA | Uniprot ID | A0A5B8R5A0 |
| Locus tag | SHEWANA3_RS21755 | Protein ID | WP_011711497.1 |
| Coordinates | 21935..22345 (+) | Length | 137 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SHEWANA3_RS21730 (Shewana3_4156) | 17444..18784 | - | 1341 | WP_011711493.1 | TolC family protein | - |
| SHEWANA3_RS23340 | 18868..19224 | - | 357 | WP_037431561.1 | hypothetical protein | - |
| SHEWANA3_RS21735 (Shewana3_4157) | 19416..20315 | - | 900 | WP_011711494.1 | cation diffusion facilitator family transporter | - |
| SHEWANA3_RS21740 (Shewana3_4158) | 20393..20800 | + | 408 | WP_011711495.1 | Cd(II)/Pb(II)-responsive transcriptional regulator | - |
| SHEWANA3_RS21745 | 20778..21074 | - | 297 | Protein_23 | iron permease | - |
| SHEWANA3_RS21750 (Shewana3_4159) | 21137..21292 | + | 156 | Protein_24 | transposase | - |
| SHEWANA3_RS21755 (Shewana3_4160) | 21935..22345 | + | 411 | WP_011711497.1 | hypothetical protein | Antitoxin |
| SHEWANA3_RS21760 (Shewana3_4161) | 22342..23283 | + | 942 | WP_011711498.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | Toxin |
| SHEWANA3_RS21765 (Shewana3_4162) | 23291..25915 | + | 2625 | WP_011711499.1 | type I restriction-modification system subunit M | - |
| SHEWANA3_RS21770 (Shewana3_4163) | 25917..27134 | + | 1218 | WP_011711500.1 | restriction endonuclease subunit S | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..278942 | 278942 | |
| - | flank | IS/Tn | - | - | 21137..21307 | 170 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 314 a.a. Molecular weight: 35771.64 Da Isoelectric Point: 7.0965
>T6033 WP_011711498.1 NC_008573:22342-23283 [Shewanella sp. ANA-3]
MKKLKQQFLEVSDALALGNPAIIEKDYWVVALLSELEQLTFETHQMVFSGGTALAKSNVKILRMSEDVDIKLIPTPQFQH
LSRGRKKQYRKALAVKIETAIIASNTFSIEARLVQDEYRYVEYELKYPQQFSQAPCLRPIIKLELMETIPLLALETRGIQ
SLVAELYKLPQEVNGFICVSVNTTLVEKVLSMLRRTMSVKRDAARSDDVTLVRHIYDVHCIHAAQALDNELLAPLFRAIL
AEDIARFGNQHAEFVMDPANELRLGLQEIESNPLFRQRFNDFVKPMVFNSQPPNFDTCFMSFKHIATSLILTI
MKKLKQQFLEVSDALALGNPAIIEKDYWVVALLSELEQLTFETHQMVFSGGTALAKSNVKILRMSEDVDIKLIPTPQFQH
LSRGRKKQYRKALAVKIETAIIASNTFSIEARLVQDEYRYVEYELKYPQQFSQAPCLRPIIKLELMETIPLLALETRGIQ
SLVAELYKLPQEVNGFICVSVNTTLVEKVLSMLRRTMSVKRDAARSDDVTLVRHIYDVHCIHAAQALDNELLAPLFRAIL
AEDIARFGNQHAEFVMDPANELRLGLQEIESNPLFRQRFNDFVKPMVFNSQPPNFDTCFMSFKHIATSLILTI
Download Length: 942 bp
>T6033 NC_008573:22342-23283 [Shewanella sp. ANA-3]
ATGAAAAAACTGAAACAACAGTTCTTAGAGGTTTCCGATGCATTAGCGCTCGGAAACCCCGCCATTATTGAAAAAGATTA
TTGGGTGGTTGCTCTACTTTCTGAGCTTGAACAGTTAACGTTTGAAACTCATCAAATGGTGTTTTCCGGCGGCACCGCAC
TGGCTAAGTCCAATGTTAAAATACTTAGAATGTCTGAAGACGTGGACATAAAGCTGATCCCCACGCCGCAATTTCAACAC
CTTAGTCGCGGCAGGAAGAAACAGTACCGAAAAGCGTTAGCAGTAAAAATTGAAACAGCGATTATTGCATCGAACACATT
TTCTATCGAAGCTAGATTAGTTCAGGATGAATATCGCTATGTGGAATATGAGCTTAAATATCCGCAACAATTTAGCCAGG
CTCCTTGTCTACGCCCAATCATCAAGTTAGAACTGATGGAAACCATTCCACTGCTTGCGCTGGAAACGCGGGGCATCCAA
TCGTTAGTCGCAGAGCTTTATAAGCTGCCACAAGAAGTGAACGGATTCATCTGCGTATCAGTAAATACCACCTTGGTAGA
GAAAGTGCTTTCCATGTTACGCCGCACAATGTCTGTTAAGCGTGATGCAGCCCGAAGCGATGACGTCACTTTAGTTAGGC
ATATTTACGACGTACACTGTATTCATGCAGCACAGGCCCTTGATAATGAATTACTTGCGCCCCTTTTTCGCGCCATATTA
GCAGAAGATATTGCTCGCTTTGGCAACCAACATGCCGAATTCGTCATGGACCCAGCCAATGAGTTACGCCTTGGCTTACA
GGAGATAGAAAGCAACCCACTCTTCAGGCAACGCTTTAATGATTTTGTTAAACCCATGGTGTTTAACTCGCAACCCCCCA
ATTTTGATACGTGCTTTATGTCCTTTAAACATATTGCGACCTCACTCATTCTGACTATTTAG
ATGAAAAAACTGAAACAACAGTTCTTAGAGGTTTCCGATGCATTAGCGCTCGGAAACCCCGCCATTATTGAAAAAGATTA
TTGGGTGGTTGCTCTACTTTCTGAGCTTGAACAGTTAACGTTTGAAACTCATCAAATGGTGTTTTCCGGCGGCACCGCAC
TGGCTAAGTCCAATGTTAAAATACTTAGAATGTCTGAAGACGTGGACATAAAGCTGATCCCCACGCCGCAATTTCAACAC
CTTAGTCGCGGCAGGAAGAAACAGTACCGAAAAGCGTTAGCAGTAAAAATTGAAACAGCGATTATTGCATCGAACACATT
TTCTATCGAAGCTAGATTAGTTCAGGATGAATATCGCTATGTGGAATATGAGCTTAAATATCCGCAACAATTTAGCCAGG
CTCCTTGTCTACGCCCAATCATCAAGTTAGAACTGATGGAAACCATTCCACTGCTTGCGCTGGAAACGCGGGGCATCCAA
TCGTTAGTCGCAGAGCTTTATAAGCTGCCACAAGAAGTGAACGGATTCATCTGCGTATCAGTAAATACCACCTTGGTAGA
GAAAGTGCTTTCCATGTTACGCCGCACAATGTCTGTTAAGCGTGATGCAGCCCGAAGCGATGACGTCACTTTAGTTAGGC
ATATTTACGACGTACACTGTATTCATGCAGCACAGGCCCTTGATAATGAATTACTTGCGCCCCTTTTTCGCGCCATATTA
GCAGAAGATATTGCTCGCTTTGGCAACCAACATGCCGAATTCGTCATGGACCCAGCCAATGAGTTACGCCTTGGCTTACA
GGAGATAGAAAGCAACCCACTCTTCAGGCAACGCTTTAATGATTTTGTTAAACCCATGGTGTTTAACTCGCAACCCCCCA
ATTTTGATACGTGCTTTATGTCCTTTAAACATATTGCGACCTCACTCATTCTGACTATTTAG
Antitoxin
Download Length: 137 a.a. Molecular weight: 15491.86 Da Isoelectric Point: 10.3652
>AT6033 WP_011711497.1 NC_008573:21935-22345 [Shewanella sp. ANA-3]
MAAVDSIFQKIKRSRRYVFERKDFDGIASYDQIGRSLNQLMDRGEIMKLGYGLYTKARVNSLTGRLMPSCPGGSDALMRE
ILKMKDIDFEIDNLSLQSLSGKNTQIPSSVHYSWDQKQFNRKLVVGNRLLTPASRP
MAAVDSIFQKIKRSRRYVFERKDFDGIASYDQIGRSLNQLMDRGEIMKLGYGLYTKARVNSLTGRLMPSCPGGSDALMRE
ILKMKDIDFEIDNLSLQSLSGKNTQIPSSVHYSWDQKQFNRKLVVGNRLLTPASRP
Download Length: 411 bp
>AT6033 NC_008573:21935-22345 [Shewanella sp. ANA-3]
ATGGCCGCTGTTGACAGTATCTTTCAAAAGATAAAGCGTTCTAGACGCTACGTTTTCGAACGCAAAGACTTTGACGGCAT
TGCTAGCTATGACCAAATTGGCCGATCGCTTAATCAGTTAATGGATAGAGGCGAGATAATGAAGCTTGGCTATGGCTTAT
ACACGAAAGCGCGAGTAAACAGCCTGACAGGACGTTTAATGCCGTCGTGTCCAGGTGGTAGCGATGCACTTATGCGTGAA
ATATTAAAAATGAAAGACATCGATTTCGAGATAGATAATCTGTCCTTACAAAGTCTTTCCGGTAAAAACACCCAAATCCC
CTCATCCGTTCATTACTCATGGGATCAAAAACAGTTCAATCGCAAATTGGTTGTTGGTAACAGACTATTAACGCCAGCAA
GTAGACCATGA
ATGGCCGCTGTTGACAGTATCTTTCAAAAGATAAAGCGTTCTAGACGCTACGTTTTCGAACGCAAAGACTTTGACGGCAT
TGCTAGCTATGACCAAATTGGCCGATCGCTTAATCAGTTAATGGATAGAGGCGAGATAATGAAGCTTGGCTATGGCTTAT
ACACGAAAGCGCGAGTAAACAGCCTGACAGGACGTTTAATGCCGTCGTGTCCAGGTGGTAGCGATGCACTTATGCGTGAA
ATATTAAAAATGAAAGACATCGATTTCGAGATAGATAATCTGTCCTTACAAAGTCTTTCCGGTAAAAACACCCAAATCCC
CTCATCCGTTCATTACTCATGGGATCAAAAACAGTTCAATCGCAAATTGGTTGTTGGTAACAGACTATTAACGCCAGCAA
GTAGACCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5B8R339 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5B8R5A0 |
References
(1) Hila Sberro et al. (2013) Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning. Molecular Cell 50(1):136-48. [PubMed:23478446]