Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1169556..1170127 Replicon chromosome
Accession NZ_CP014161
Organism Aerococcus urinae strain CCUG36881

Toxin (Protein)


Gene name mazF Uniprot ID A0A0X8FET5
Locus tag AWM73_RS05345 Protein ID WP_060778411.1
Coordinates 1169795..1170127 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A120I9U0
Locus tag AWM73_RS05340 Protein ID WP_060778410.1
Coordinates 1169556..1169801 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWM73_RS05325 1166010..1166903 - 894 WP_144435190.1 IS3 family transposase -
AWM73_RS05330 1166899..1167558 - 660 WP_144435191.1 helix-turn-helix domain-containing protein -
AWM73_RS05335 1167985..1168476 - 492 WP_060785031.1 hypothetical protein -
AWM73_RS09060 1168592..1168735 - 144 WP_156417358.1 hypothetical protein -
AWM73_RS05340 1169556..1169801 + 246 WP_060778410.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AWM73_RS05345 1169795..1170127 + 333 WP_060778411.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AWM73_RS05350 1170238..1171404 - 1167 WP_060778412.1 nucleotide sugar dehydrogenase -
AWM73_RS05355 1171524..1172939 - 1416 WP_174519269.1 lipopolysaccharide biosynthesis protein -
AWM73_RS05360 1173009..1173920 - 912 WP_060778414.1 glycosyltransferase -
AWM73_RS05365 1173929..1174861 - 933 WP_060778415.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - ugd 1157068..1177246 20178


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12735.81 Da        Isoelectric Point: 9.2408

>T60318 WP_060778411.1 NZ_CP014161:1169795-1170127 [Aerococcus urinae]
MVKVKQGSIIKINLDPKQGHEQKGYRPYICLSHGIVTKYSNIAIFAPISNTKRKYPFYVELEQTKTTGKVLLDQLVTIDF
NARDYNYIEQVTDDILIDLLARVKVLFEKE

Download         Length: 333 bp

>T60318 NZ_CP014161:1169795-1170127 [Aerococcus urinae]
ATGGTAAAAGTGAAGCAAGGATCGATTATCAAAATAAATTTAGATCCAAAACAGGGACATGAGCAAAAAGGCTATAGGCC
CTATATTTGTTTGAGCCATGGTATTGTTACTAAGTACTCAAATATTGCCATTTTTGCACCGATTAGCAATACAAAAAGAA
AATATCCCTTTTATGTAGAGTTAGAACAGACAAAAACAACTGGAAAAGTACTCTTAGATCAGTTAGTTACCATCGATTTT
AATGCCAGAGACTATAATTATATCGAACAAGTCACAGATGATATCCTCATTGATTTATTAGCTAGGGTAAAAGTATTATT
CGAAAAAGAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9325.69 Da        Isoelectric Point: 4.7863

>AT60318 WP_060778410.1 NZ_CP014161:1169556-1169801 [Aerococcus urinae]
MPLTINKWGNSSAIRLPKQLLEELNLSQGDKLDYQVKDGQLIIKKITEVPELTVKDLFENYDGAPVNEKPVLFEQRGNEK
W

Download         Length: 246 bp

>AT60318 NZ_CP014161:1169556-1169801 [Aerococcus urinae]
ATGCCTTTAACAATTAATAAATGGGGGAATAGTTCTGCTATTCGATTACCAAAACAGTTGCTTGAAGAATTAAATTTATC
CCAAGGCGACAAACTGGATTATCAAGTGAAAGATGGTCAACTGATTATAAAAAAAATAACCGAAGTTCCTGAACTTACGG
TTAAAGATTTGTTTGAAAATTATGATGGAGCTCCTGTTAATGAAAAGCCCGTATTATTTGAACAAAGAGGAAATGAAAAA
TGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X8FET5

Antitoxin

Source ID Structure
AlphaFold DB A0A120I9U0

References