Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF-MazE
Location 102915..103504 Replicon chromosome
Accession NZ_CP014161
Organism Aerococcus urinae strain CCUG36881

Toxin (Protein)


Gene name pemK Uniprot ID A0A0X8FD65
Locus tag AWM73_RS00450 Protein ID WP_060777568.1
Coordinates 103151..103504 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0X8FCZ2
Locus tag AWM73_RS00445 Protein ID WP_060777567.1
Coordinates 102915..103157 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWM73_RS00410 98172..98717 + 546 WP_060777561.1 hypothetical protein -
AWM73_RS00415 98765..99379 + 615 WP_060777562.1 hypothetical protein -
AWM73_RS00420 99405..99911 + 507 WP_060777563.1 hypothetical protein -
AWM73_RS00425 100044..101180 + 1137 WP_060777564.1 hypothetical protein -
AWM73_RS00430 101354..101578 + 225 WP_174519266.1 hypothetical protein -
AWM73_RS00435 101619..101966 + 348 WP_060777565.1 hypothetical protein -
AWM73_RS00440 102092..102649 - 558 WP_060777566.1 isochorismatase family protein -
AWM73_RS00445 102915..103157 + 243 WP_060777567.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AWM73_RS00450 103151..103504 + 354 WP_060777568.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AWM73_RS00455 103732..104109 + 378 WP_060777569.1 GntR family transcriptional regulator -
AWM73_RS00460 104102..104791 + 690 WP_060777570.1 ABC transporter ATP-binding protein -
AWM73_RS00465 104788..105594 + 807 WP_060777571.1 hypothetical protein -
AWM73_RS00470 105607..106758 + 1152 WP_060777572.1 hypothetical protein -
AWM73_RS00475 107055..108449 + 1395 WP_060777573.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-99)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13876.81 Da        Isoelectric Point: 8.0664

>T60316 WP_060777568.1 NZ_CP014161:103151-103504 [Aerococcus urinae]
MVMRQGDIFYVNFNPNIGHEQRNSRPAIVLSHDLIFQTSRMAIVAPISTTKRNYPAYYELQETDHTKGKVLLDQTKSLDL
YHCQVSQENFIERAKPNEFKRIIHRYKLLFDLVDDYQ

Download         Length: 354 bp

>T60316 NZ_CP014161:103151-103504 [Aerococcus urinae]
ATGGTAATGCGGCAGGGCGACATTTTTTACGTTAATTTTAATCCAAATATCGGGCACGAACAGAGAAATAGTCGTCCGGC
TATTGTATTAAGTCATGATCTCATTTTTCAGACGAGTCGAATGGCGATTGTTGCGCCTATCTCAACAACTAAACGAAATT
ATCCAGCCTATTATGAGCTTCAAGAGACTGACCATACCAAGGGTAAAGTTCTTTTGGATCAAACAAAGTCTCTTGATCTT
TATCATTGCCAAGTCAGCCAAGAAAATTTTATTGAACGAGCTAAACCGAATGAATTTAAGCGTATTATTCATCGTTATAA
ATTGTTATTCGATCTGGTAGATGATTATCAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9213.44 Da        Isoelectric Point: 4.1954

>AT60316 WP_060777567.1 NZ_CP014161:102915-103157 [Aerococcus urinae]
MQTKIAQWGNSKAIRIPSTIVKQLNLKDNQVLSLEIKDQALVIKPKEAETIQELFADYNTSFTYEEEMDWGPAVGEEIEW

Download         Length: 243 bp

>AT60316 NZ_CP014161:102915-103157 [Aerococcus urinae]
ATGCAAACTAAAATTGCTCAATGGGGGAATTCAAAGGCGATCAGAATCCCATCAACGATAGTTAAACAGCTCAATTTAAA
GGATAATCAAGTGCTCTCACTTGAAATCAAAGATCAGGCCCTAGTGATTAAGCCTAAGGAAGCAGAAACTATTCAGGAAT
TATTTGCTGATTACAACACTTCTTTCACTTATGAGGAAGAAATGGATTGGGGACCAGCAGTGGGAGAAGAGATCGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X8FD65

Antitoxin

Source ID Structure
AlphaFold DB A0A0X8FCZ2

References