Detailed information of TA system TA060314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 573991..574580
• Replicon: chromosome
• Accession: NZ_CP014160
• Organism: Aerococcus sanguinicola strain CCUG43001

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AWM72_RS02560
• Protein ID: WP_067976528.1
• Coordinates: 573991..574341 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0X8FAC4
• Locus tag: AWM72_RS02565
• Protein ID: WP_067972694.1
• Coordinates: 574338..574580 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWM72_RS02540	569496..570686	-	1191	WP_067972682.1	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase	-
AWM72_RS02545	570895..571644	-	750	WP_067972685.1	SDR family oxidoreductase	-
AWM72_RS02550	571799..572671	+	873	WP_067972688.1	LysR family transcriptional regulator	-
AWM72_RS02555	572731..573678	-	948	WP_067972691.1	hypothetical protein	-
AWM72_RS02560	573991..574341	-	351	WP_067976528.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWM72_RS02565	574338..574580	-	243	WP_067972694.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AWM72_RS02570	574722..576263	-	1542	WP_067972697.1	ATP-binding cassette domain-containing protein	-
AWM72_RS02580	576803..577108	-	306	WP_067972700.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
AWM72_RS02585	577314..578351	-	1038	WP_067972702.1	dienelactone hydrolase family protein	-
AWM72_RS02590	578558..578812	+	255	WP_067972706.1	prevent-host-death protein	-
AWM72_RS02595	578816..579130	+	315	WP_067972708.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-93)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13584.53 Da
• Isoelectric Point: 9.2217

>T60314 WP_067976528.1 NZ_CP014160:c574341-573991 [Aerococcus sanguinicola]
VMRQGDIFYVNFNPSIGHEQRSSRPAIVLSHDLIFKTSRMAIVAPISTTKRNYPAYYELQETEHIKGKVLLDQSKALDLY
HRQVSQADFIERAKPNEFKGIIHRYKLLFDLVDDYQ

Nucleotide

• Length: 351 bp

>T60314 NZ_CP014160:c574341-573991 [Aerococcus sanguinicola]
GTGATGCGGCAGGGAGATATTTTTTATGTTAATTTTAATCCAAGTATCGGACACGAGCAGAGAAGTAGTCGCCCAGCTAT
TGTATTGAGTCATGATCTTATCTTCAAGACGAGTCGCATGGCAATCGTTGCGCCTATCTCAACAACTAAACGAAATTATC
CAGCCTATTATGAGCTTCAAGAAACTGAACATATCAAGGGTAAAGTTCTTTTAGATCAATCAAAGGCTCTTGATCTTTAT
CACCGTCAAGTCAGTCAAGCAGATTTCATTGAACGAGCTAAGCCAAATGAATTTAAAGGTATTATTCATCGCTATAAATT
GTTATTTGATCTGGTAGATGACTATCAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9243.51 Da
• Isoelectric Point: 4.3228

>AT60314 WP_067972694.1 NZ_CP014160:c574580-574338 [Aerococcus sanguinicola]
MQTKLAQWGNSKAIRIPSTIVKQLDLKNNQVLSLEIKDQSLVIKPKEAETIQELFADYKTSFTYEEEMDWGPAVGEELEW

Nucleotide

• Length: 243 bp

>AT60314 NZ_CP014160:c574580-574338 [Aerococcus sanguinicola]
ATGCAAACCAAACTTGCTCAATGGGGGAATTCAAAGGCGATCAGAATTCCATCAACTATAGTTAAACAACTCGATTTAAA
GAATAACCAAGTGCTCTCACTCGAAATCAAAGATCAAAGCTTAGTGATTAAGCCTAAGGAAGCAGAGACGATTCAGGAAT
TATTTGCTGATTACAAGACATCTTTCACTTATGAAGAAGAGATGGATTGGGGGCCAGCAGTGGGAGAAGAGCTCGAATGG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0X8FAC4