Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 6122832..6123406 Replicon chromosome
Accession NZ_CP014158
Organism Pseudomonas citronellolis strain P3B5

Toxin (Protein)


Gene name vapC Uniprot ID A0A1S1GEF4
Locus tag PcP3B5_RS26820 Protein ID WP_043268833.1
Coordinates 6122832..6123197 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID W5ISP5
Locus tag PcP3B5_RS26825 Protein ID WP_009613188.1
Coordinates 6123191..6123406 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PcP3B5_RS26800 6118376..6120787 + 2412 WP_061562846.1 TonB-dependent receptor -
PcP3B5_RS26805 6120795..6121943 + 1149 WP_061562847.1 PepSY domain-containing protein -
PcP3B5_RS26810 6121940..6122122 + 183 WP_061562848.1 hypothetical protein -
PcP3B5_RS26815 6122119..6122844 + 726 WP_061562849.1 hypothetical protein -
PcP3B5_RS26820 6122832..6123197 - 366 WP_043268833.1 type II toxin-antitoxin system VapC family toxin Toxin
PcP3B5_RS26825 6123191..6123406 - 216 WP_009613188.1 ribbon-helix-helix domain-containing protein Antitoxin
PcP3B5_RS26830 6123475..6125499 - 2025 WP_061562850.1 oligopeptide transporter, OPT family -
PcP3B5_RS26835 6125625..6126461 - 837 WP_043268829.1 taurine dioxygenase -
PcP3B5_RS26840 6126649..6127467 - 819 WP_061562851.1 ABC transporter permease subunit -
PcP3B5_RS26845 6127457..6128248 - 792 WP_043318713.1 ATP-binding cassette domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13390.48 Da        Isoelectric Point: 5.8613

>T60312 WP_043268833.1 NZ_CP014158:c6123197-6122832 [Pseudomonas citronellolis]
MVKALFDTNILIDYLNGHEQARDELRRYDDPAISIVTWMEVMIGATPATAAATRAFLDSFALVPLDAPVAERAVAVRQAL
RVKLPDAIIKASAEVQGRLLVTRNTRDFPVDDAGVRLPYRL

Download         Length: 366 bp

>T60312 NZ_CP014158:c6123197-6122832 [Pseudomonas citronellolis]
ATGGTGAAGGCGCTGTTCGACACCAACATCCTCATCGACTACCTCAACGGCCACGAGCAGGCCCGCGACGAGTTGCGCCG
CTACGATGACCCCGCCATCAGCATCGTCACCTGGATGGAGGTGATGATCGGCGCCACGCCCGCCACCGCCGCGGCCACCC
GCGCCTTCCTCGACAGCTTCGCCCTGGTGCCGCTCGACGCGCCCGTCGCCGAGCGCGCCGTGGCGGTGCGCCAGGCGCTG
CGGGTGAAGCTGCCGGACGCCATCATCAAGGCCAGCGCCGAAGTGCAGGGGCGCCTGCTGGTCACCCGCAACACCAGGGA
CTTCCCCGTCGACGACGCCGGCGTGCGCCTGCCCTACCGGCTCTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8033.94 Da        Isoelectric Point: 4.7616

>AT60312 WP_009613188.1 NZ_CP014158:c6123406-6123191 [Pseudomonas citronellolis]
MRTLVDIPAEHLEALNALGEQQQQSRAALIREAVAEYLVRHRRQDAEAAFGLWNGSAGDGLAYQEKVREEW

Download         Length: 216 bp

>AT60312 NZ_CP014158:c6123406-6123191 [Pseudomonas citronellolis]
ATGCGTACCCTGGTCGACATCCCCGCCGAACATCTCGAAGCGCTCAATGCCCTGGGCGAGCAACAGCAGCAATCGCGCGC
CGCGCTGATCCGCGAGGCGGTCGCCGAGTATCTGGTCCGGCACCGCCGACAGGATGCCGAGGCCGCCTTCGGCCTGTGGA
ACGGCAGCGCCGGCGACGGCCTGGCCTACCAGGAGAAGGTGCGCGAGGAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S1GEF4

Antitoxin

Source ID Structure
AlphaFold DB A0A1A5D6Z2

References