Detailed information of TA system TA006031

Experimentally validated

Overview

TA module

• Type: IV
• Classification (family/domain): sdenTA/-
• Location: 332784..334224
• Replicon: chromosome
• Accession: NC_007954
• Organism: Shewanella denitrificans OS217

Toxin (Protein)

• Gene name: sdenT
• Uniprot ID: Q12SI1
• Locus tag: SDEN_RS01590
• Protein ID: WP_011494762.1
• Coordinates: 333337..334224 (+)
• Length: 296 a.a.

Antitoxin (Protein)

• Gene name: sdenA
• Uniprot ID: Q12SI2
• Locus tag: SDEN_RS01585
• Protein ID: WP_011494761.1
• Coordinates: 332784..333308 (+)
• Length: 175 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SDEN_RS20425 (Sden_0295)	328075..328351	-	277	Protein_304	DUF4143 domain-containing protein	-
SDEN_RS01570 (Sden_0296)	328711..330219	+	1509	WP_011494758.1	hypothetical protein	-
SDEN_RS01575 (Sden_0297)	330281..330820	-	540	Protein_306	Fic family protein	-
SDEN_RS20430	330901..331041	-	141	WP_157599809.1	hypothetical protein	-
SDEN_RS01580 (Sden_0298)	331141..332454	-	1314	WP_011494760.1	ATP-binding protein	-
SDEN_RS01585 (Sden_0299)	332784..333308	+	525	WP_011494761.1	hypothetical protein	Antitoxin
SDEN_RS01590 (Sden_0300)	333337..334224	+	888	WP_011494762.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
SDEN_RS20435	334184..334462	-	279	WP_157599810.1	hypothetical protein	-
SDEN_RS01600 (Sden_0301)	334795..337950	+	3156	WP_011494763.1	DUF3427 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-270)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 296 a.a.
• Molecular weight: 34029.00 Da
• Isoelectric Point: 5.7514

>T6031 WP_011494762.1 NC_007954:333337-334224 [Shewanella denitrificans OS217]
MLKQQIRQIVQANPDYAAVTPVIEKEILHHDIMDVLIKQGVMQRLTFIGGTSLRMCYNSSRLSEDLDFNGGHDFKPSEFD
GLESEIQTYIQNKYETEVWVNKPSGEQQGDTVSWKISIIKEANRPDLPRQKMHIDVCAIPSFDIEKRPLLNHYDIVVPTE
GILVPVQSLQETLADKLIALAYRARRIKPRDIWDIVWIKQRGINLSKTLVEKKLVARQKQTEDFRQALSVQLGKLMTEDE
VRNDFNMEMSRFIPKQIKERTLDNPEYWAYVQAEVNAIASELLNDGTAKNPFDMG

Nucleotide

• Length: 888 bp

>T6031 NC_007954:333337-334224 [Shewanella denitrificans OS217]
ATGTTGAAGCAGCAAATTCGTCAAATCGTCCAAGCAAACCCTGACTATGCAGCAGTGACACCTGTTATAGAAAAAGAGAT
CTTGCATCATGACATTATGGATGTGCTGATCAAACAGGGTGTTATGCAGCGATTGACCTTTATTGGTGGCACCTCATTAC
GTATGTGCTACAACAGCAGCAGACTGTCGGAAGACCTTGATTTTAACGGCGGTCATGATTTCAAACCCTCGGAGTTTGAT
GGGTTGGAGTCCGAAATCCAAACCTATATTCAGAACAAGTATGAAACAGAAGTTTGGGTCAACAAACCTTCCGGCGAGCA
GCAGGGGGATACAGTTTCTTGGAAAATTAGTATTATAAAAGAGGCTAATCGACCTGATTTGCCTCGGCAAAAAATGCATA
TTGACGTCTGTGCTATTCCGTCATTCGATATCGAAAAGCGCCCGTTACTCAATCATTACGACATCGTTGTACCGACAGAA
GGGATCTTGGTTCCGGTACAGTCTTTACAAGAAACACTGGCTGATAAGCTGATCGCGTTAGCGTATCGCGCAAGGCGCAT
AAAGCCTCGTGATATATGGGATATTGTTTGGATCAAGCAACGCGGCATTAACTTATCAAAGACACTGGTTGAAAAAAAAC
TCGTCGCCAGACAGAAGCAAACCGAAGATTTTCGTCAAGCACTGTCTGTACAGTTGGGCAAACTGATGACAGAAGATGAA
GTACGTAACGACTTCAACATGGAAATGAGCCGCTTTATCCCTAAGCAAATTAAAGAGAGAACCCTAGATAATCCTGAATA
CTGGGCGTATGTTCAGGCTGAGGTAAATGCCATCGCGTCTGAATTACTTAATGACGGAACAGCAAAAAATCCATTTGATA
TGGGTTAG

Antitoxin

Protein

• Length: 175 a.a.
• Molecular weight: 19575.89 Da
• Isoelectric Point: 9.6665

>AT6031 WP_011494761.1 NC_007954:332784-333308 [Shewanella denitrificans OS217]
MAKSDELLNVLAKAVKAGGGIHSATELAFMLGVPCDPAFRKFLTDCVKKRRLRRVIKGFYESVITPPEPETAIYKIIKKL
RSGVLNYISLESQLSHTGDISQIVMGRVTVVTKGRSGCFDTPYGVIEFTHTKKPIEQVAPNLYYDPDIKMYRAYKEQAIA
DLKHCQRNLHMLES

Nucleotide

• Length: 525 bp

>AT6031 NC_007954:332784-333308 [Shewanella denitrificans OS217]
ATGGCAAAATCAGATGAGCTATTAAACGTATTAGCAAAAGCGGTTAAAGCGGGCGGCGGCATTCACAGTGCAACTGAGCT
GGCTTTTATGCTTGGTGTGCCTTGTGACCCAGCCTTTAGAAAGTTCCTAACAGATTGCGTAAAGAAAAGACGACTAAGAC
GAGTGATTAAAGGTTTCTATGAAAGCGTTATTACACCTCCTGAGCCTGAGACTGCCATTTATAAAATTATCAAAAAGCTG
CGAAGTGGCGTATTGAACTACATCAGCCTCGAAAGCCAGCTCAGTCATACAGGCGATATTTCACAAATCGTGATGGGCAG
AGTTACTGTGGTGACTAAAGGTCGAAGTGGTTGTTTTGATACGCCCTATGGCGTGATCGAATTTACCCATACTAAAAAGC
CAATCGAGCAAGTTGCTCCCAATTTGTACTATGACCCCGATATCAAAATGTACCGAGCGTACAAAGAACAAGCGATTGCT
GACCTAAAACACTGTCAGCGCAATCTACATATGTTGGAGAGCTAA

Structures

Toxin

• AlphaFold DB: Q12SI1

Antitoxin

• AlphaFold DB: Q12SI2

References

(1) Hila Sberro et al. (2013) Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning. Molecular Cell 50(1):136-48. [PubMed:23478446]


 experimental literature