Detailed information of TA system TA060304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2295991..2296580
• Replicon: chromosome
• Accession: NZ_CP014158
• Organism: Pseudomonas citronellolis strain P3B5

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: PcP3B5_RS10445
• Protein ID: WP_061561203.1
• Coordinates: 2295991..2296347 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A127MR37
• Locus tag: PcP3B5_RS10450
• Protein ID: WP_061561204.1
• Coordinates: 2296341..2296580 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PcP3B5_RS10420	2290998..2292092	+	1095	WP_061561199.1	D-alanine--D-alanine ligase	-
PcP3B5_RS10425	2292114..2292464	-	351	WP_058070122.1	hypothetical protein	-
PcP3B5_RS10430	2292888..2293316	+	429	WP_145923872.1	hypothetical protein	-
PcP3B5_RS10435	2293554..2294147	+	594	WP_061561201.1	histidine phosphatase family protein	-
PcP3B5_RS10440	2294239..2295048	+	810	WP_061561202.1	class I SAM-dependent methyltransferase	-
PcP3B5_RS10445	2295991..2296347	-	357	WP_061561203.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PcP3B5_RS10450	2296341..2296580	-	240	WP_061561204.1	hypothetical protein	Antitoxin
PcP3B5_RS10455	2296716..2297321	-	606	WP_061561205.1	LysE family translocator	-
PcP3B5_RS10460	2297373..2298209	-	837	WP_058489028.1	AraC family transcriptional regulator	-
PcP3B5_RS10465	2298290..2299906	-	1617	WP_203228545.1	hypothetical protein	-
PcP3B5_RS31955	2299921..2300271	-	351	WP_203228560.1	peptidoglycan-binding protein	-
PcP3B5_RS10475	2300657..2301280	-	624	WP_009618994.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13115.95 Da
• Isoelectric Point: 7.3242

>T60304 WP_061561203.1 NZ_CP014158:c2296347-2295991 [Pseudomonas citronellolis]
MVRRPPSVPDRGEIWHLQFDPSTGREMIGDHFCLVLSKREFNEIGLALVCPISQGASNVARNQGFLVTLMGEGTDTQGNV
HSHQVRTLDWRARRAAFKEKAPDSVTNYVLSLVGAILE

Nucleotide

• Length: 357 bp

>T60304 NZ_CP014158:c2296347-2295991 [Pseudomonas citronellolis]
ATGGTGAGGCGTCCGCCGAGCGTTCCCGACCGCGGTGAGATCTGGCACTTGCAGTTCGATCCCTCGACGGGCCGTGAAAT
GATCGGCGACCACTTTTGCCTGGTCCTGTCGAAACGCGAATTTAATGAAATAGGCCTGGCCCTGGTCTGCCCCATCTCGC
AAGGCGCATCCAATGTGGCGCGCAACCAGGGCTTCCTTGTCACCCTGATGGGCGAAGGCACCGACACCCAGGGCAATGTC
CACAGCCACCAGGTGCGGACGCTGGACTGGAGGGCGCGCCGAGCCGCATTCAAGGAAAAGGCTCCCGATTCCGTCACCAA
CTACGTGCTTTCGCTGGTCGGGGCGATCCTGGAGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8326.63 Da
• Isoelectric Point: 5.6980

>AT60304 WP_061561204.1 NZ_CP014158:c2296580-2296341 [Pseudomonas citronellolis]
MTTLALRKSGGSLIISVPSAFVAQNKLHAGSQVECNIVEGALVIKPITRPSLAQLIAETPKDSLAPDWDSMPAVGAEQW

Nucleotide

• Length: 240 bp

>AT60304 NZ_CP014158:c2296580-2296341 [Pseudomonas citronellolis]
ATGACCACGCTTGCCCTGCGCAAATCCGGGGGGTCCCTGATCATTTCGGTTCCCTCGGCCTTCGTCGCCCAGAACAAACT
GCACGCCGGCAGTCAGGTTGAGTGCAACATCGTTGAGGGGGCCCTGGTGATCAAGCCGATAACGCGCCCGAGCCTGGCCC
AGCTGATCGCCGAAACGCCCAAAGACTCGCTCGCCCCGGACTGGGACAGCATGCCGGCCGTAGGCGCGGAACAATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A127MR37