Detailed information of TA system TA060302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 1446512..1447120
• Replicon: chromosome
• Accession: NZ_CP014158
• Organism: Pseudomonas citronellolis strain P3B5

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A1S1GNG8
• Locus tag: PcP3B5_RS06580
• Protein ID: WP_043272446.1
• Coordinates: 1446512..1446859 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: PcP3B5_RS30540
• Protein ID: WP_081884705.1
• Coordinates: 1446869..1447120 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PcP3B5_RS06565	1443269..1443943	-	675	WP_009618249.1	DUF4194 domain-containing protein	-
PcP3B5_RS06570	1443975..1445447	-	1473	WP_009618251.1	DUF3375 domain-containing protein	-
PcP3B5_RS06575	1445543..1446148	-	606	WP_009618253.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
PcP3B5_RS06580	1446512..1446859	-	348	WP_043272446.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PcP3B5_RS30540	1446869..1447120	-	252	WP_081884705.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PcP3B5_RS06585	1447577..1448992	+	1416	WP_061560835.1	amino acid permease	-
PcP3B5_RS06590	1449118..1449654	+	537	WP_061560836.1	2,4'-dihydroxyacetophenone dioxygenase family protein	-
PcP3B5_RS06595	1449711..1450616	+	906	WP_061560837.1	LysR family transcriptional regulator	-
PcP3B5_RS06600	1450679..1451920	+	1242	WP_061560838.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12935.75 Da
• Isoelectric Point: 4.6337

>T60302 WP_043272446.1 NZ_CP014158:c1446859-1446512 [Pseudomonas citronellolis]
MSKVVIRLTDTAEQSIEDQVHHLAQFQGSQAALQSVLSLLDEIEEKISLTPQGYPISQQASLLGVLNYRELNTGPYRVFY
ELHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIG

Nucleotide

• Length: 348 bp

>T60302 NZ_CP014158:c1446859-1446512 [Pseudomonas citronellolis]
ATGTCCAAGGTCGTCATCCGTCTCACCGATACCGCAGAACAAAGCATCGAAGACCAAGTACACCACTTGGCTCAATTCCA
AGGTTCGCAGGCTGCACTCCAATCGGTGCTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCTCTTACACCCCAAGGTT
ACCCAATCAGCCAGCAGGCAAGCCTTTTGGGGGTGTTGAACTATCGCGAACTCAATACCGGCCCTTATCGCGTCTTTTAC
GAACTACACGAAGAGCAAGGCGAGGTCGCGGTTATCCTGGTCTTACGCCAGAAGCAGAGTGTGGAACAGCAATTGATTCG
CTACTGCTTGGTGGGGCCTATCGGGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9500.82 Da
• Isoelectric Point: 8.4753

>AT60302 WP_081884705.1 NZ_CP014158:c1447120-1446869 [Pseudomonas citronellolis]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVIESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFD
QPE

Nucleotide

• Length: 252 bp

>AT60302 NZ_CP014158:c1447120-1446869 [Pseudomonas citronellolis]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGCCATGCGGCCGACCTGGATTTATCCGAGCCAATGGTCGTCACACAGAA
CGGTGTTCCTGCCTACGTGATTGAGTCATATGCAGAACGAAAGCAACGTGACGAAGCAATTGCGTTGGTGAAGCTGCTTG
CGATTGGCTCTCGTCAATACGCAGAAGGCAAACATCGCTCTGTAGATGATCTGAAAGCTCGTCTTTCCAGGAGGTTCGAT
CAACCAGAATAA

Structures

Toxin

• AlphaFold DB: A0A1S1GNG8