Detailed information of TA system TA060301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1139717..1140186
• Replicon: chromosome
• Accession: NZ_CP014158
• Organism: Pseudomonas citronellolis strain P3B5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PcP3B5_RS30520
• Protein ID: WP_024128153.1
• Coordinates: 1139717..1139992 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W5IQM6
• Locus tag: PcP3B5_RS05255
• Protein ID: WP_009617620.1
• Coordinates: 1139992..1140186 (-)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PcP3B5_RS05215	1135429..1136727	-	1299	WP_061560719.1	NAD(P)/FAD-dependent oxidoreductase	-
PcP3B5_RS05220	1136774..1136947	-	174	WP_009617611.1	DUF3094 domain-containing protein	-
PcP3B5_RS05225	1137017..1137646	-	630	WP_009617612.1	DUF1780 domain-containing protein	-
PcP3B5_RS05230	1137779..1138195	+	417	WP_058071484.1	GNAT family N-acetyltransferase	-
PcP3B5_RS05235	1138198..1138674	+	477	WP_061560720.1	hypothetical protein	-
PcP3B5_RS05240	1138671..1139387	+	717	WP_061560721.1	energy-coupling factor ABC transporter permease	-
PcP3B5_RS05245	1139495..1139710	+	216	WP_061560722.1	hypothetical protein	-
PcP3B5_RS30520	1139717..1139992	-	276	WP_024128153.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
PcP3B5_RS05255	1139992..1140186	-	195	WP_009617620.1	hypothetical protein	Antitoxin
PcP3B5_RS05260	1140452..1140661	-	210	WP_043272268.1	DNA gyrase inhibitor YacG	-
PcP3B5_RS05265	1140658..1141266	-	609	WP_009617624.1	dephospho-CoA kinase	-
PcP3B5_RS05270	1141340..1142212	-	873	WP_061560723.1	A24 family peptidase	-
PcP3B5_RS05275	1142213..1143430	-	1218	WP_061560724.1	type II secretion system F family protein	-
PcP3B5_RS05280	1143434..1145137	-	1704	WP_061560725.1	type IV-A pilus assembly ATPase PilB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10478.03 Da
• Isoelectric Point: 7.3551

>T60301 WP_024128153.1 NZ_CP014158:c1139992-1139717 [Pseudomonas citronellolis]
VLSIIWRRSASDDLATILAYIANEDPQAARRLKERVESAVLPLAQHPYLYRHGRVPGTREVVIHPNYILVYRIDAECIEV
VSVLHSRQEYP

Nucleotide

• Length: 276 bp

>T60301 NZ_CP014158:c1139992-1139717 [Pseudomonas citronellolis]
GTGCTATCGATCATCTGGCGCAGGAGTGCCAGTGATGACCTCGCGACCATTCTCGCCTATATAGCCAACGAGGATCCCCA
AGCAGCAAGGCGCCTCAAGGAACGCGTGGAGTCTGCCGTGCTGCCGTTGGCCCAGCATCCCTACCTTTACCGTCATGGAC
GAGTACCCGGCACACGAGAAGTCGTGATCCACCCCAACTACATCCTGGTTTACCGGATCGACGCTGAATGCATCGAAGTA
GTCAGCGTCCTGCATTCGCGCCAGGAATATCCCTGA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7293.07 Da
• Isoelectric Point: 4.9890

>AT60301 WP_009617620.1 NZ_CP014158:c1140186-1139992 [Pseudomonas citronellolis]
MSARLSPIVSEFETPEQAASHDRWFRQKVQKSLTDNQPGRPHDEVMVDMDAIIAQAEARRQGND

Nucleotide

• Length: 195 bp

>AT60301 NZ_CP014158:c1140186-1139992 [Pseudomonas citronellolis]
ATGAGCGCCCGACTTTCCCCCATAGTCTCCGAGTTCGAAACCCCGGAGCAGGCAGCCAGCCATGATCGCTGGTTCCGCCA
AAAGGTGCAGAAGTCCCTTACAGACAACCAACCAGGGCGTCCCCATGACGAGGTAATGGTCGACATGGACGCCATCATTG
CCCAGGCCGAAGCCCGGCGCCAGGGCAACGACTAG

Structures

Antitoxin

• AlphaFold DB: A0A1A5D6N4