Detailed information of TA system TA060299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 716064..716683
• Replicon: chromosome
• Accession: NZ_CP014158
• Organism: Pseudomonas citronellolis strain P3B5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W5J3G4
• Locus tag: PcP3B5_RS03205
• Protein ID: WP_009615522.1
• Coordinates: 716064..716246 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PcP3B5_RS03210
• Protein ID: WP_043319209.1
• Coordinates: 716279..716683 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PcP3B5_RS30370	714658..714816	+	159	WP_009615518.1	YqaE/Pmp3 family membrane protein	-
PcP3B5_RS03200	715117..715605	-	489	WP_061560561.1	Bro-N domain-containing protein	-
PcP3B5_RS03205	716064..716246	+	183	WP_009615522.1	type II toxin-antitoxin system HicA family toxin	Toxin
PcP3B5_RS03210	716279..716683	+	405	WP_043319209.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PcP3B5_RS03215	716726..717910	-	1185	WP_061560562.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	715117..737948	22831

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6962.13 Da
• Isoelectric Point: 12.2993

>T60299 WP_009615522.1 NZ_CP014158:716064-716246 [Pseudomonas citronellolis]
MRSREIMELIRADGWFLVEVKGSHHQFRHFTKKGRVTVPHPRSNLPIGTVNSILRQAGLR

Nucleotide

• Length: 183 bp

>T60299 NZ_CP014158:716064-716246 [Pseudomonas citronellolis]
ATGCGCAGTCGAGAAATCATGGAGTTGATCCGGGCGGATGGCTGGTTCCTGGTTGAGGTGAAAGGCAGTCATCACCAGTT
CCGGCATTTCACCAAGAAGGGGCGGGTGACGGTTCCCCACCCCAGGAGCAACCTGCCGATCGGGACCGTGAACAGCATTC
TGCGCCAGGCCGGCCTGCGATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14505.21 Da
• Isoelectric Point: 4.5714

>AT60299 WP_043319209.1 NZ_CP014158:716279-716683 [Pseudomonas citronellolis]
MKFPVVLHKDADSDYGVTIPDVPGCFSAGGTVSQALENVQEALALHFEGLVADGETLPQAQEVDTHMGNPDYAGGVWAVV
DFDVTPYLGKAVRFNASLPENLLQRIDERVKRDHRYASRSGFLATAALRELSSS

Nucleotide

• Length: 405 bp

>AT60299 NZ_CP014158:716279-716683 [Pseudomonas citronellolis]
ATGAAATTCCCCGTGGTTCTGCACAAGGACGCCGACTCGGATTACGGCGTGACCATCCCTGACGTGCCTGGCTGCTTCTC
CGCCGGCGGCACCGTCAGCCAGGCGCTGGAAAACGTCCAGGAAGCCCTGGCCCTGCACTTCGAAGGCCTGGTGGCCGACG
GCGAGACGCTCCCGCAAGCCCAGGAAGTGGACACCCACATGGGCAACCCCGACTACGCCGGCGGCGTCTGGGCGGTGGTC
GACTTCGACGTCACCCCCTACCTGGGCAAGGCCGTGCGCTTCAACGCCAGCCTCCCGGAAAACCTCCTGCAACGCATCGA
CGAACGCGTGAAGCGCGACCATCGCTACGCCTCCCGCTCCGGCTTCCTGGCTACCGCCGCGCTGCGTGAGTTGTCTTCGA
GCTGA

Structures

Toxin

• AlphaFold DB: A0A127MLP4