Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4021174..4021793 Replicon chromosome
Accession NZ_CP014156
Organism Klebsiella quasipneumoniae strain HKUOPL4

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AWV60_RS19225 Protein ID WP_002892050.1
Coordinates 4021174..4021392 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AWV60_RS19230 Protein ID WP_002892066.1
Coordinates 4021419..4021793 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWV60_RS19195 4017218..4017481 + 264 WP_004204754.1 type B 50S ribosomal protein L31 -
AWV60_RS19200 4017481..4017621 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AWV60_RS19205 4017618..4018316 - 699 WP_053810701.1 GNAT family N-acetyltransferase -
AWV60_RS19210 4018417..4019874 + 1458 WP_049117056.1 PLP-dependent aminotransferase family protein -
AWV60_RS19215 4019843..4020313 - 471 WP_049093338.1 YlaC family protein -
AWV60_RS24540 4020356..4020478 + 123 WP_032431198.1 hypothetical protein -
AWV60_RS19220 4020450..4021016 - 567 WP_004204750.1 maltose O-acetyltransferase -
AWV60_RS19225 4021174..4021392 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AWV60_RS19230 4021419..4021793 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AWV60_RS19235 4022278..4025424 - 3147 WP_017898892.1 multidrug efflux RND transporter permease subunit -
AWV60_RS19240 4025447..4026640 - 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T60296 WP_002892050.1 NZ_CP014156:c4021392-4021174 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T60296 NZ_CP014156:c4021392-4021174 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT60296 WP_002892066.1 NZ_CP014156:c4021793-4021419 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT60296 NZ_CP014156:c4021793-4021419 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References