Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/- |
| Location | 1348442..1349358 | Replicon | chromosome |
| Accession | NC_000964 | ||
| Organism | Bacillus subtilis subsp. subtilis str. 168 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | O34853 |
| Locus tag | BSU_12830 | Protein ID | NP_389166.1 |
| Coordinates | 1348612..1349358 (-) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | O34800 |
| Locus tag | BSU_12820 | Protein ID | NP_389165.1 |
| Coordinates | 1348442..1348612 (-) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BSU_12760 (BSU12760) | 1345305..1345634 | + | 330 | NP_389159.1 | phage PBSX; conserved hypothetical protein | - |
| BSU_12770 (BSU12770) | 1345631..1345795 | + | 165 | NP_389160.1 | phage PBSX; conserved hypothetical protein | - |
| BSU_12780 (BSU12780) | 1345839..1346678 | + | 840 | NP_389161.2 | phage PBSX; lytic exoenzyme | - |
| BSU_12790 (BSU12790) | 1346731..1347000 | + | 270 | NP_389162.1 | phage PBSX; putative enzyme | - |
| BSU_12800 (BSU12800) | 1347013..1347276 | + | 264 | NP_389163.1 | phage PBSX; holin | - |
| BSU_12810 (BSU12810) | 1347289..1348182 | + | 894 | NP_389164.1 | phage PBSX; N-acetylmuramoyl-L-alanine amidase | - |
| BSU_12815 (BSU12815) | 1348219..1348356 | - | 138 | YP_009513952.1 | three component toxin / antitoxin / antitoxin SpoIISABC, antitoxin C | - |
| BSU_12820 (BSU12820) | 1348442..1348612 | - | 171 | NP_389165.1 | three-component apoptotic control system component B (antitoxin B) | Antitoxin |
| BSU_12830 (BSU12830) | 1348612..1349358 | - | 747 | NP_389166.1 | three-component apoptosis factor (ABC toxin/antitoxin/antitoxin system) | Toxin |
| BSU_12840 (BSU12840) | 1349468..1350469 | - | 1002 | NP_389167.2 | putative low-affinity inorganic phosphate transporter | - |
| BSU_12850 (BSU12850) | 1350482..1351099 | - | 618 | NP_389168.1 | putative PitB transporter accessory protein | - |
| BSU_12860 (BSU12860) | 1351375..1352691 | - | 1317 | NP_389169.1 | serine / threonine exchanger | - |
| BSU_12870 (BSU12870) | 1353080..1354030 | + | 951 | NP_389170.1 | hydroquinone-specific extradiol dioxygenase | - |
| BSU_12875 (BSU12875) | 1354131..1354277 | + | 147 | YP_009513953.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29061.50 Da Isoelectric Point: 4.6191
>T6029 NP_389166.1 NC_000964:c1349358-1348612 [Bacillus subtilis subsp. subtilis str. 168]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
Download Length: 747 bp
>T6029 NC_000964:c1349358-1348612 [Bacillus subtilis subsp. subtilis str. 168]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6654.72 Da Isoelectric Point: 11.0203
>AT6029 NP_389165.1 NC_000964:c1348612-1348442 [Bacillus subtilis subsp. subtilis str. 168]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
Download Length: 171 bp
>AT6029 NC_000964:c1348612-1348442 [Bacillus subtilis subsp. subtilis str. 168]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) E Adler et al. (2001) Bacillus subtilis locus encoding a killer protein and its antidote. Journal of Bacteriology 183(12):3574-81. [PubMed:11371520]