Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 207859..208498 Replicon chromosome
Accession NZ_CP014150
Organism Paeniclostridium sordellii strain AM370

Toxin (Protein)


Gene name mazF Uniprot ID A0A446IEP0
Locus tag RSJ16_RS01500 Protein ID WP_021130257.1
Coordinates 208133..208498 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A446IEN4
Locus tag RSJ16_RS01495 Protein ID WP_021126762.1
Coordinates 207859..208143 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RSJ16_RS01465 203327..204721 + 1395 WP_101508069.1 F0F1 ATP synthase subunit beta -
RSJ16_RS01470 204724..204984 + 261 WP_021130261.1 ATP synthase F1 subunit epsilon -
RSJ16_RS01475 205186..205566 + 381 WP_021126768.1 holo-ACP synthase -
RSJ16_RS01480 205572..206033 + 462 WP_021126767.1 CBS domain-containing protein -
RSJ16_RS01485 206059..206649 + 591 WP_101508070.1 hypothetical protein -
RSJ16_RS01490 206665..207828 + 1164 WP_055330943.1 alanine racemase -
RSJ16_RS01495 207859..208143 + 285 WP_021126762.1 hypothetical protein Antitoxin
RSJ16_RS01500 208133..208498 + 366 WP_021130257.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RSJ16_RS01505 208664..209491 + 828 WP_021130256.1 transketolase -
RSJ16_RS01510 209484..210416 + 933 WP_021130255.1 transketolase family protein -
RSJ16_RS01515 210544..210960 + 417 WP_055332920.1 hypothetical protein -
RSJ16_RS01520 211089..213470 + 2382 WP_057567256.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-118)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13469.61 Da        Isoelectric Point: 8.0080

>T60263 WP_021130257.1 NZ_CP014150:208133-208498 [Paeniclostridium sordellii]
VSSNSLDIKRGHLYYADLSPVVGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHIEISSNEYGLNKDSVI
LLEQIRTIDKKRLREKIGYLDECMMEKVNNSLLISLGLFQL

Download         Length: 366 bp

>T60263 NZ_CP014150:208133-208498 [Paeniclostridium sordellii]
GTGAGTTCTAATAGTTTAGATATCAAACGCGGACATTTATATTATGCAGATTTAAGCCCTGTTGTTGGATCTGAACAAGG
TGGTATAAGACCAGTACTCATAATACAAAATGATATAGGTAATAAATACAGTCCAACAGTTATAGTTGCAGCTATAACAT
CTCAAATAAACAAGGCAAAATTACCAACTCATATAGAAATAAGTTCTAATGAGTATGGGCTTAATAAAGATTCCGTGATT
CTCTTAGAACAAATTCGAACGATAGATAAAAAAAGATTGAGAGAAAAAATAGGTTATCTAGATGAATGTATGATGGAAAA
AGTAAATAATAGTCTATTAATTAGTTTAGGATTATTTCAACTTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10700.15 Da        Isoelectric Point: 4.4179

>AT60263 WP_021126762.1 NZ_CP014150:207859-208143 [Paeniclostridium sordellii]
VRNIGKEKIVFNLPDSLISEVDHIVQIENTNREDFAKAAFQFYITQKKKFDLKESMIKGYKEMGQINLTLAELGMTNEVS
SDDDLEGIIAQGEF

Download         Length: 285 bp

>AT60263 NZ_CP014150:207859-208143 [Paeniclostridium sordellii]
GTGCGCAATATTGGTAAGGAAAAAATTGTATTTAATTTACCTGATTCTCTGATCTCAGAAGTAGATCATATAGTTCAAAT
AGAGAATACTAATAGAGAAGACTTTGCAAAGGCTGCTTTTCAATTCTACATTACTCAAAAGAAAAAATTTGATCTTAAAG
AATCCATGATAAAAGGATACAAAGAAATGGGTCAAATAAATTTAACTTTAGCCGAGCTAGGAATGACAAATGAGGTATCT
TCTGATGATGACCTTGAAGGGATTATAGCTCAAGGTGAGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A446IEP0

Antitoxin

Source ID Structure
AlphaFold DB A0A446IEN4

References