Detailed information of TA system TA060256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1827238..1827724
• Replicon: chromosome
• Accession: NZ_CP014139
• Organism: Streptococcus pyogenes strain MEW123

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q48QR0
• Locus tag: AWM59_RS08850
• Protein ID: WP_014407961.1
• Coordinates: 1827238..1827507 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A660A1J7
• Locus tag: AWM59_RS08855
• Protein ID: WP_014407962.1
• Coordinates: 1827497..1827724 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWM59_RS08835	1822733..1823806	+	1074	WP_011285298.1	DUF4097 family beta strand repeat protein	-
AWM59_RS08840	1823923..1826196	-	2274	WP_021340697.1	YhgE/Pip domain-containing protein	-
AWM59_RS08845	1826331..1826864	+	534	WP_010922785.1	TetR/AcrR family transcriptional regulator	-
AWM59_RS08850	1827238..1827507	-	270	WP_014407961.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AWM59_RS08855	1827497..1827724	-	228	WP_014407962.1	hypothetical protein	Antitoxin
AWM59_RS08860	1827877..1828248	-	372	WP_011285302.1	DUF1492 domain-containing protein	-
AWM59_RS08865	1828238..1828585	-	348	WP_011285303.1	DUF1492 domain-containing protein	-
AWM59_RS08870	1828865..1829110	-	246	WP_011285304.1	hypothetical protein	-
AWM59_RS08875	1829085..1829360	-	276	WP_014407964.1	hypothetical protein	-
AWM59_RS08880	1829350..1829688	-	339	WP_030126557.1	replication protein	-
AWM59_RS08885	1829730..1829995	-	266	Protein_1693	hypothetical protein	-
AWM59_RS08890	1829992..1830780	-	789	WP_011285308.1	hypothetical protein	-
AWM59_RS09880	1830871..1831029	-	159	WP_011285309.1	hypothetical protein	-
AWM59_RS08895	1831043..1831288	-	246	WP_011285310.1	hypothetical protein	-
AWM59_RS08900	1831284..1831610	-	327	Protein_1697	hypothetical protein	-
AWM59_RS09885	1831612..1831785	-	174	WP_011285312.1	hypothetical protein	-
AWM59_RS09555	1831791..1831964	-	174	WP_000694580.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10497.50 Da
• Isoelectric Point: 10.5027

>T60256 WP_014407961.1 NZ_CP014139:c1827507-1827238 [Streptococcus pyogenes]
MTYKLVVSDEVKKQLKKMDKHVGLMLAKDMKKRLDGLNNPRQFGKALTGQYKGLWRYRVGNYRVICDIVDNEMIILALEV
GHRKEIYKR

Nucleotide

• Length: 270 bp

>T60256 NZ_CP014139:c1827507-1827238 [Streptococcus pyogenes]
ATGACTTATAAGCTAGTGGTGAGTGATGAAGTCAAGAAGCAACTCAAAAAGATGGATAAACATGTAGGGCTTATGTTAGC
CAAAGACATGAAGAAACGACTTGACGGGTTAAATAATCCTAGACAATTTGGAAAAGCCCTCACAGGTCAATATAAAGGAC
TTTGGCGCTATCGTGTTGGTAATTATCGGGTGATTTGTGACATTGTGGATAATGAAATGATTATCCTTGCTTTGGAAGTT
GGACACAGGAAAGAGATATACAAGCGCTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8641.63 Da
• Isoelectric Point: 3.9607

>AT60256 WP_014407962.1 NZ_CP014139:c1827724-1827497 [Streptococcus pyogenes]
MAMVSLRLNSAEEELFRSYATHTGKTLSELFKTALAQQIEDQLDYETGIQALKRFEENPITYSIDDLIEELENDL

Nucleotide

• Length: 228 bp

>AT60256 NZ_CP014139:c1827724-1827497 [Streptococcus pyogenes]
ATGGCAATGGTATCATTAAGACTGAACAGCGCAGAGGAAGAACTTTTCAGAAGCTACGCTACACACACAGGCAAAACATT
ATCTGAATTATTCAAGACAGCACTAGCACAACAAATTGAAGATCAATTGGACTATGAAACAGGTATTCAAGCTTTAAAAC
GCTTTGAAGAAAATCCCATAACTTATTCTATCGATGATTTAATTGAGGAGTTGGAAAATGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A660A202

Antitoxin

• AlphaFold DB: A0A660A1J7