Detailed information of TA system TA060250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3306078..3306603
• Replicon: chromosome
• Accession: NZ_CP014137
• Organism: Brenneria goodwinii strain FRB141

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AWC36_RS14705
• Protein ID: WP_048636106.1
• Coordinates: 3306319..3306603 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C6D956
• Locus tag: AWC36_RS14700
• Protein ID: WP_005973616.1
• Coordinates: 3306078..3306329 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC36_RS14680	3301260..3301505	+	246	WP_095834786.1	helix-turn-helix domain-containing protein	-
AWC36_RS14685	3302297..3303172	+	876	WP_095834787.1	integrase domain-containing protein	-
AWC36_RS14690	3303467..3305071	+	1605	WP_095834788.1	ATP-binding protein	-
AWC36_RS14695	3305576..3305827	+	252	Protein_2855	winged helix-turn-helix domain-containing protein	-
AWC36_RS14700	3306078..3306329	+	252	WP_005973616.1	prevent-host-death protein	Antitoxin
AWC36_RS14705	3306319..3306603	+	285	WP_048636106.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AWC36_RS14710	3307047..3307244	+	198	WP_095835813.1	integrase	-
AWC36_RS14715	3307251..3308516	-	1266	WP_095834789.1	alpha-hydroxy-acid oxidizing protein	-
AWC36_RS14720	3308927..3310891	+	1965	WP_048636108.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3301260..3315008	13748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11030.01 Da
• Isoelectric Point: 10.3023

>T60250 WP_048636106.1 NZ_CP014137:3306319-3306603 [Brenneria goodwinii]
MSYKLEFEERALKEFKKLGAPVREQFTKKLKEVLQNPHVPANRLHGMADCYKIKLRSAGYRLVYQVIEHEIVVLVLAVSK
RERSEVYKAAKDRL

Nucleotide

• Length: 285 bp

>T60250 NZ_CP014137:3306319-3306603 [Brenneria goodwinii]
ATGAGTTATAAGCTGGAGTTTGAAGAGCGCGCGCTGAAAGAATTTAAGAAGCTGGGCGCACCCGTGCGGGAGCAGTTCAC
CAAAAAACTGAAAGAGGTTTTACAAAATCCCCACGTTCCCGCCAATCGTCTTCATGGAATGGCTGACTGCTACAAAATCA
AACTTCGTTCCGCTGGCTATCGGTTAGTTTATCAAGTGATTGAGCATGAGATCGTGGTGCTGGTTTTAGCTGTGAGCAAA
CGGGAACGCTCAGAAGTTTATAAAGCAGCGAAAGACCGACTATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9079.35 Da
• Isoelectric Point: 5.0807

>AT60250 WP_005973616.1 NZ_CP014137:3306078-3306329 [Brenneria goodwinii]
MSVQPILANAAVSISDFKKSPNAALKEANGEPVAVLTNGRISGYYVSPETWEALADYQEDIELAKIARSRMKGKRVKVDL
DEL

Nucleotide

• Length: 252 bp

>AT60250 NZ_CP014137:3306078-3306329 [Brenneria goodwinii]
ATGTCTGTACAACCTATCCTTGCTAATGCTGCCGTGAGTATCAGCGACTTTAAAAAATCGCCTAATGCCGCGCTTAAAGA
AGCTAACGGCGAACCTGTAGCCGTGTTAACTAATGGCCGTATCTCTGGCTATTATGTTTCCCCCGAAACATGGGAAGCTC
TGGCCGACTATCAGGAAGATATTGAACTCGCCAAAATTGCCCGTTCACGGATGAAGGGGAAACGTGTGAAGGTCGATCTG
GATGAGTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0G4JQP6