Detailed information of TA system TA060249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3136690..3137216
• Replicon: chromosome
• Accession: NZ_CP014137
• Organism: Brenneria goodwinii strain FRB141

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: AWC36_RS14025
• Protein ID: WP_095834717.1
• Coordinates: 3136911..3137216 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: AWC36_RS14020
• Protein ID: WP_015855076.1
• Coordinates: 3136690..3136908 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC36_RS14005	3132939..3135092	-	2154	WP_095834716.1	ornithine decarboxylase	-
AWC36_RS14015	3136158..3136610	+	453	Protein_2722	DUF4102 domain-containing protein	-
AWC36_RS14020	3136690..3136908	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
AWC36_RS14025	3136911..3137216	+	306	WP_095834717.1	type II toxin-antitoxin system toxin CcdB	Toxin
AWC36_RS14030	3137355..3138398	-	1044	WP_095834718.1	methyltransferase	-
AWC36_RS14035	3138450..3139457	-	1008	WP_095834719.1	HlyD family secretion protein	-
AWC36_RS14040	3139551..3140570	-	1020	WP_048635980.1	NADP-dependent oxidoreductase	-
AWC36_RS14045	3140641..3141264	-	624	WP_095834720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11626.55 Da
• Isoelectric Point: 6.7166

>T60249 WP_095834717.1 NZ_CP014137:3136911-3137216 [Brenneria goodwinii]
MQFIVYKYKRASHYKMFVDVQSDIIETPGRRMVIPFIESHHLSEKVNKALFPPIRINGENYRLMTTELSSVPVEVIGETI
ADLSNCADEIKNAINLMLWGI

Nucleotide

• Length: 306 bp

>T60249 NZ_CP014137:3136911-3137216 [Brenneria goodwinii]
ATGCAATTCATTGTTTATAAATACAAAAGAGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGTGATATTATTGAGAC
GCCTGGGCGGCGAATGGTCATTCCGTTCATAGAGTCACATCACCTGTCTGAGAAAGTGAATAAAGCGCTGTTCCCCCCGA
TCCGCATCAACGGCGAAAATTATCGGCTGATGACCACTGAATTATCGAGCGTTCCCGTTGAGGTCATTGGTGAAACTATC
GCGGACCTCAGCAACTGCGCGGATGAGATCAAAAATGCGATTAACCTTATGCTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT60249 WP_015855076.1 NZ_CP014137:3136690-3136908 [Brenneria goodwinii]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT60249 NZ_CP014137:3136690-3136908 [Brenneria goodwinii]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGTCT
GGTGAACGATGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAAGAGTGGAAGAAGGAAAACCGTGAAGGAATGGAGG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46