Detailed information of TA system TA060248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2918440..2919068
• Replicon: chromosome
• Accession: NZ_CP014137
• Organism: Brenneria goodwinii strain FRB141

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E0SD87
• Locus tag: AWC36_RS13080
• Protein ID: WP_009114000.1
• Coordinates: 2918865..2919068 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: AWC36_RS13075
• Protein ID: WP_048635795.1
• Coordinates: 2918440..2918808 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC36_RS13060	2913933..2917067	+	3135	WP_048635793.1	efflux RND transporter permease subunit	-
AWC36_RS13065	2917262..2917510	+	249	WP_048635794.1	type B 50S ribosomal protein L31	-
AWC36_RS13070	2917711..2917854	+	144	WP_072065916.1	type B 50S ribosomal protein L36	-
AWC36_RS13075	2918440..2918808	+	369	WP_048635795.1	Hha toxicity modulator TomB	Antitoxin
AWC36_RS13080	2918865..2919068	+	204	WP_009114000.1	hemolysin expression modulator Hha	Toxin
AWC36_RS13090	2919858..2920178	+	321	WP_048635796.1	MGMT family protein	-
AWC36_RS13095	2920208..2920783	-	576	WP_048635797.1	YbaY family lipoprotein	-
AWC36_RS13100	2921039..2921902	+	864	WP_048635798.1	acyl-CoA thioesterase II	-
AWC36_RS13105	2921994..2923280	-	1287	WP_048635799.1	ammonium transporter AmtB	-
AWC36_RS13110	2923317..2923655	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8133.51 Da
• Isoelectric Point: 8.8500

>T60248 WP_009114000.1 NZ_CP014137:2918865-2919068 [Brenneria goodwinii]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Nucleotide

• Length: 204 bp

>T60248 NZ_CP014137:2918865-2919068 [Brenneria goodwinii]
ATGAAAAAAATCGATTATTTGATGCGTTTACGTAAATGCACAACCATAGACACATTGGAACGTGTTATTGAGAAAAACAA
GTATGAGCTCTCCAACGATGAACTGGAGATGTTCTATTCTGCGGCGGATCATCGTCTGGCAGAACTGACGATGAACAAGC
TATACGACAAAGTTCCCACCGCGGTATGGAAATATGTCCGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14066.78 Da
• Isoelectric Point: 4.3233

>AT60248 WP_048635795.1 NZ_CP014137:2918440-2918808 [Brenneria goodwinii]
MDEYTPKNYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPDENELSQRVEKYL
DDTYVLFSNYGINDAELRGWKKSKERLFGMFSEGDICTPAKT

Nucleotide

• Length: 369 bp

>AT60248 NZ_CP014137:2918440-2918808 [Brenneria goodwinii]
ATGGATGAGTACACACCCAAAAATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTATGCGACGAAAGCATTGC
TACGTTAGGCGATAGCAGCCACGGCTGGGTCAATGACCCGACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACATTTATTCTCACATTTAAAATAAAATATCCTGACGAAAACGAGCTTTCTCAGCGGGTTGAAAAGTATTTG
GATGACACTTATGTCCTGTTTAGCAACTATGGCATCAATGATGCTGAATTGCGCGGATGGAAGAAATCTAAAGAAAGATT
ATTCGGAATGTTCTCAGAAGGGGACATCTGTACGCCTGCTAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A1X3RNQ0