Detailed information of TA system TA060236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3715973..3717009
• Replicon: chromosome
• Accession: NZ_CP014136
• Organism: Gibbsiella quercinecans strain FRB97

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: AWC35_RS17215
• Protein ID: WP_095847440.1
• Coordinates: 3716434..3717009 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: AWC35_RS17210
• Protein ID: WP_095847439.1
• Coordinates: 3715973..3716434 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC35_RS17185	3711259..3712017	-	759	WP_095847434.1	flap endonuclease Xni	-
AWC35_RS17190	3712112..3713479	-	1368	WP_095847435.1	LOG family protein	-
AWC35_RS17195	3713676..3714521	-	846	WP_095847436.1	NADPH-dependent 7-cyano-7-deazaguanine reductase QueF	-
AWC35_RS17200	3714584..3715135	+	552	WP_095847437.1	SecY-interacting protein	-
AWC35_RS17210	3715973..3716434	+	462	WP_095847439.1	helix-turn-helix domain-containing protein	Antitoxin
AWC35_RS17215	3716434..3717009	+	576	WP_095847440.1	PIN domain-containing protein	Toxin
AWC35_RS17220	3717291..3718112	+	822	WP_095847441.1	AraC family transcriptional regulator	-
AWC35_RS17225	3718083..3718721	-	639	WP_095847442.1	LysE family transporter	-
AWC35_RS17230	3718911..3719420	+	510	WP_095847443.1	GNAT family N-acetyltransferase	-
AWC35_RS17235	3719512..3720750	-	1239	WP_095847444.1	MFS transporter	-
AWC35_RS17240	3720924..3721253	+	330	WP_165907093.1	YqcC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21676.07 Da
• Isoelectric Point: 4.7194

>T60236 WP_095847440.1 NZ_CP014136:3716434-3717009 [Gibbsiella quercinecans]
MTLSPYPVVLDACVLYPSLLRDLLMHLGLTGLYQPKWSAIIEEEWQRNLIKDRPDLNSDQIKQTGELMNRALPDAMVTGF
EPLVASIDLPDVDDRHVVAAAICSNAEIVVTFNLKDFPLQYLNNFGIEALHPDDFITDFFDLNQALVLAAVNKQRRNMRK
PPKSVDEYLDALLRQALPQTVKELSKFRALI

Nucleotide

• Length: 576 bp

>T60236 NZ_CP014136:3716434-3717009 [Gibbsiella quercinecans]
ATGACTCTTTCACCTTATCCCGTAGTATTAGATGCTTGCGTACTCTATCCATCCTTACTACGGGATTTATTGATGCATCT
GGGACTGACCGGGCTGTATCAACCGAAATGGTCGGCCATTATCGAAGAAGAATGGCAGCGTAATTTGATTAAGGATAGAC
CTGACTTAAATTCTGACCAAATCAAACAAACTGGGGAGCTTATGAATCGGGCATTGCCTGATGCAATGGTTACAGGTTTC
GAGCCGCTCGTTGCCAGTATCGATCTTCCTGATGTTGATGACAGGCATGTTGTTGCTGCCGCCATTTGCAGCAATGCTGA
AATCGTCGTTACGTTCAACTTGAAAGATTTCCCACTACAATACCTGAATAATTTCGGCATTGAAGCATTACATCCAGATG
ATTTTATTACTGATTTTTTCGATTTGAATCAGGCACTTGTTCTGGCGGCCGTAAACAAGCAGCGCCGCAATATGAGAAAG
CCGCCGAAATCGGTTGATGAGTATCTTGATGCGCTACTTCGCCAGGCTCTACCACAAACAGTAAAAGAATTGAGTAAATT
CCGCGCCCTTATTTAG

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17077.52 Da
• Isoelectric Point: 6.7621

>AT60236 WP_095847439.1 NZ_CP014136:3715973-3716434 [Gibbsiella quercinecans]
MKNSILDSLSLPAQGEIEAAVRGQRELAAYLSTKMETQKISIQDADNNTRQIELPTSSLTLLMSILGELAVGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEDGKLPFHKTGRHRRVLFADLMRYKDQRDSESHKAMQVLADHSQELGFY

Nucleotide

• Length: 462 bp

>AT60236 NZ_CP014136:3715973-3716434 [Gibbsiella quercinecans]
ATGAAAAACTCAATTCTTGATAGCCTGAGCCTTCCGGCTCAAGGGGAGATTGAGGCAGCTGTTCGTGGGCAAAGGGAGCT
TGCTGCCTACCTCTCCACGAAAATGGAAACACAAAAAATTTCGATTCAGGACGCAGATAACAACACTCGCCAGATCGAGT
TGCCAACGTCTTCATTGACGCTGCTTATGTCGATTTTAGGAGAGTTGGCCGTCGGTAACGCGGTTCAGGTTGTTCCTGTG
CATGCTGAATTAACAACGCAGGAAGCTGCCAACATTCTGAATGTTTCTCGCCCTCATATGGTGAAACTACTGGAAGATGG
GAAGCTACCTTTTCACAAAACAGGTCGCCATCGCCGCGTGCTTTTCGCCGATTTAATGAGGTACAAAGATCAGCGAGATA
GCGAAAGCCACAAAGCAATGCAGGTATTGGCAGATCATAGCCAGGAACTTGGATTTTACTGA