Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1490880..1491457 Replicon chromosome
Accession NZ_CP014136
Organism Gibbsiella quercinecans strain FRB97

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag AWC35_RS06975 Protein ID WP_095845669.1
Coordinates 1491125..1491457 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag AWC35_RS06970 Protein ID WP_095845668.1
Coordinates 1490880..1491125 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWC35_RS06945 1486145..1486738 - 594 WP_183096718.1 lipid IV(A) palmitoyltransferase PagP -
AWC35_RS06950 1487338..1488057 + 720 WP_095845665.1 DUF554 domain-containing protein -
AWC35_RS06955 1488062..1489162 - 1101 WP_095848942.1 DUF1615 domain-containing protein -
AWC35_RS06960 1489344..1490201 + 858 WP_095845666.1 SAM-dependent methyltransferase TehB -
AWC35_RS06965 1490270..1490911 - 642 WP_165907129.1 ATP-binding cassette domain-containing protein -
AWC35_RS06970 1490880..1491125 + 246 WP_095845668.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AWC35_RS06975 1491125..1491457 + 333 WP_095845669.1 endoribonuclease MazF Toxin
AWC35_RS06980 1491669..1491833 + 165 Protein_1349 IS110 family transposase -
AWC35_RS06985 1491826..1492197 + 372 Protein_1350 transposase -
AWC35_RS06990 1492242..1492514 - 273 WP_095845670.1 hypothetical protein -
AWC35_RS06995 1492532..1494043 - 1512 WP_095845671.1 sugar ABC transporter ATP-binding protein -
AWC35_RS07000 1494094..1495317 - 1224 WP_095845672.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1491832..1492197 365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11896.77 Da        Isoelectric Point: 6.9251

>T60232 WP_095845669.1 NZ_CP014136:1491125-1491457 [Gibbsiella quercinecans]
MVSRFVPDAGDLIWLDFDPVEGHEQGGRRPAVVLSPFAYNNKVGLLLCVPCTTKAKDYPFEVEIAGDRDGVALADQVTCI
DWRARKVAKKGIVSAAELADIRAKAKALIG

Download         Length: 333 bp

>T60232 NZ_CP014136:1491125-1491457 [Gibbsiella quercinecans]
ATGGTATCGCGTTTTGTACCTGATGCCGGTGATTTAATCTGGCTAGACTTCGATCCGGTAGAAGGGCATGAGCAGGGCGG
ACGCCGACCAGCTGTTGTCTTGTCACCTTTTGCCTACAACAACAAGGTTGGGCTATTGCTGTGCGTTCCATGCACGACAA
AGGCTAAGGATTATCCATTTGAGGTGGAAATCGCGGGTGATAGGGACGGCGTGGCACTGGCGGATCAGGTGACATGCATA
GACTGGCGGGCAAGGAAGGTTGCCAAAAAAGGGATAGTCAGTGCTGCCGAACTTGCAGATATCAGGGCTAAAGCAAAAGC
GCTGATCGGATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8682.10 Da        Isoelectric Point: 5.0022

>AT60232 WP_095845668.1 NZ_CP014136:1490880-1491125 [Gibbsiella quercinecans]
MAQSVVKKWGNSPSVRLPAAIMKAAALNVDDTVDIVVENGRIVIVPVRAKEYSLDALLVGITDENLHEKVDFGGPVGKEL
I

Download         Length: 246 bp

>AT60232 NZ_CP014136:1490880-1491125 [Gibbsiella quercinecans]
ATGGCGCAGTCAGTGGTTAAAAAATGGGGCAATAGCCCATCTGTAAGGTTGCCAGCGGCGATTATGAAAGCAGCAGCGCT
GAATGTTGATGATACTGTGGATATTGTCGTAGAAAATGGCCGTATTGTGATCGTTCCGGTACGAGCGAAAGAGTATTCGT
TAGATGCGTTGTTAGTTGGAATAACTGATGAAAATCTGCATGAGAAAGTGGATTTTGGTGGACCGGTTGGGAAGGAATTG
ATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References