Detailed information of TA system TA060231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1394442..1395063
• Replicon: chromosome
• Accession: NZ_CP014136
• Organism: Gibbsiella quercinecans strain FRB97

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: AWC35_RS06520
• Protein ID: WP_095845586.1
• Coordinates: 1394860..1395063 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: AWC35_RS06515
• Protein ID: WP_095845585.1
• Coordinates: 1394442..1394810 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC35_RS06480	1390533..1390787	+	255	WP_095845579.1	type B 50S ribosomal protein L31	-
AWC35_RS06485	1390799..1390939	+	141	WP_073529259.1	type B 50S ribosomal protein L36	-
AWC35_RS06490	1391040..1391918	-	879	WP_095845580.1	metal ABC transporter substrate-binding protein	-
AWC35_RS06495	1391946..1392803	-	858	WP_095845581.1	metal ABC transporter permease	-
AWC35_RS06500	1392800..1393510	-	711	WP_095845582.1	ABC transporter ATP-binding protein	-
AWC35_RS06510	1393929..1394282	+	354	WP_095845584.1	hypothetical protein	-
AWC35_RS06515	1394442..1394810	+	369	WP_095845585.1	Hha toxicity modulator TomB	Antitoxin
AWC35_RS06520	1394860..1395063	+	204	WP_095845586.1	hemolysin expression modulator Hha	Toxin
AWC35_RS06530	1395889..1396203	+	315	WP_095845587.1	MGMT family protein	-
AWC35_RS06535	1396247..1396741	-	495	WP_095845588.1	YbaY family lipoprotein	-
AWC35_RS06540	1396969..1397835	+	867	WP_095845589.1	acyl-CoA thioesterase II	-
AWC35_RS06545	1397920..1399206	-	1287	WP_121525299.1	ammonium transporter AmtB	-
AWC35_RS06550	1399245..1399583	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8116.42 Da
• Isoelectric Point: 6.9756

>T60231 WP_095845586.1 NZ_CP014136:1394860-1395063 [Gibbsiella quercinecans]
MTKIDYLMRLRKCTNLDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTAVWKYVR

Nucleotide

• Length: 204 bp

>T60231 NZ_CP014136:1394860-1395063 [Gibbsiella quercinecans]
ATGACAAAGATCGACTATTTGATGCGGTTACGTAAATGCACCAACCTTGACACGCTGGAGCGTGTTATTGAAAAAAATAA
ATACGAGCTTTCCGATGACGAGCTTGAGCTGTTTTATTCCGCAGCCGATCACCGCCTGGCGGAGCTCACCATGAATAAGC
TGTATGATAAAATCCCTACCGCAGTCTGGAAATACGTACGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14075.70 Da
• Isoelectric Point: 4.4085

>AT60231 WP_095845585.1 NZ_CP014136:1394442-1394810 [Gibbsiella quercinecans]
MDEYSPKRHDIAQLKFLCENLYDEGLATIGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESELSDLVEEYL
DDTYTLFSNYGINDSDLGRWQKTKARLFRMFSGEGTCTAMKS

Nucleotide

• Length: 369 bp

>AT60231 NZ_CP014136:1394442-1394810 [Gibbsiella quercinecans]
ATGGATGAGTATTCGCCTAAGCGGCACGATATTGCTCAACTGAAGTTCTTGTGTGAGAATCTTTATGATGAGGGCCTCGC
GACAATAGGCGACAGCCATCATGGTTGGGTGAACGATCCAACGTCTTCCGTCAATCTCCAGCTTAACGAGTTGATCGAAC
ATATTGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAAAGCGAACTCTCGGATCTGGTCGAAGAGTATCTC
GACGACACTTATACCCTTTTTAGTAATTACGGTATTAATGATTCAGACTTGGGCCGTTGGCAAAAAACCAAGGCACGATT
ATTCAGAATGTTCTCAGGAGAGGGCACCTGTACGGCAATGAAATCTTAA