Detailed information of TA system TA060217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 84850..85760
• Replicon: plasmid unnamed2
• Accession: NZ_CP014127
• Organism: Pantoea vagans strain FDAARGOS_160

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AL522_RS00940
• Protein ID: WP_061060614.1
• Coordinates: 85290..85760 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AL522_RS00935
• Protein ID: WP_061060613.1
• Coordinates: 84850..85293 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL522_RS00910	80106..80831	-	726	WP_010246779.1	ABC transporter permease subunit	-
AL522_RS00915	80838..81686	-	849	WP_013195994.1	transporter substrate-binding domain-containing protein	-
AL522_RS00920	81708..82895	-	1188	WP_061060610.1	amidohydrolase	-
AL522_RS00925	83170..84036	-	867	WP_061060611.1	alpha/beta hydrolase	-
AL522_RS00930	84088..84702	-	615	WP_061060612.1	TetR/AcrR family transcriptional regulator	-
AL522_RS00935	84850..85293	+	444	WP_061060613.1	DUF2384 domain-containing protein	Antitoxin
AL522_RS00940	85290..85760	+	471	WP_061060614.1	RES family NAD+ phosphorylase	Toxin
AL522_RS00945	85933..86964	+	1032	WP_061060615.1	AI-2E family transporter	-
AL522_RS00950	86953..89682	-	2730	WP_061060616.1	cation-transporting P-type ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla / icmF/tssM / tssF / iroN	1..470309	470309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (32-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17409.80 Da
• Isoelectric Point: 5.1978

>T60217 WP_061060614.1 NZ_CP014127:85290-85760 [Pantoea vagans]
MILYRLTKTKYLSTAWTGFGAKEAGGRWNSIGTSMVYVSETASLTLLETLVHLHSAHILDAFTLLRIDVPDALIQTVDIS
ELPANWADEEAPAELADYGDAWCSACDAVALRVPSALSPIEHNYLLNPEHPNFSRIVQQAEAIPFRFDRRLKPDRK

Nucleotide

• Length: 471 bp

>T60217 NZ_CP014127:85290-85760 [Pantoea vagans]
TTGATCCTCTACAGGCTGACTAAGACAAAGTATCTTTCGACAGCCTGGACGGGATTCGGCGCAAAAGAGGCGGGCGGCCG
CTGGAACAGCATCGGCACCTCAATGGTTTATGTCTCAGAAACAGCCTCACTGACGTTGCTGGAAACACTGGTCCATCTGC
ACTCAGCACATATCCTCGACGCTTTCACCCTACTGCGCATCGATGTTCCCGATGCGCTGATACAAACCGTCGATATCAGT
GAATTGCCTGCAAACTGGGCCGATGAAGAAGCACCGGCGGAACTGGCTGACTATGGCGACGCCTGGTGCTCTGCCTGCGA
TGCGGTTGCGTTGCGTGTACCCAGTGCCCTCTCGCCCATAGAACACAACTATCTGTTAAATCCTGAACACCCGAATTTTT
CCCGGATCGTTCAACAGGCTGAGGCCATTCCGTTCCGGTTTGACCGTCGGCTGAAACCGGACCGTAAATAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16444.82 Da
• Isoelectric Point: 9.5907

>AT60217 WP_061060613.1 NZ_CP014127:84850-85293 [Pantoea vagans]
MKTFSFSAAKTRPPRLWQAAGLHSTDGVALLGQINDGLDGNVAHLIIDWAKITQNDLRRMSGIPSTTFSRGVKAKFTAEQ
SERLVRIIRVIDRAVELFEGDKDEAQKWLNEPNRALSWKMPAELMASETGAYEVLKLITRLENGVYS

Nucleotide

• Length: 444 bp

>AT60217 NZ_CP014127:84850-85293 [Pantoea vagans]
ATGAAAACATTCAGCTTTTCTGCAGCTAAGACCAGACCGCCACGCTTATGGCAGGCTGCGGGCCTGCACAGCACGGATGG
TGTCGCTCTGCTGGGCCAGATCAACGATGGCCTTGATGGCAACGTGGCTCACCTCATCATCGACTGGGCGAAGATTACCC
AAAACGACCTGCGCAGAATGTCCGGTATTCCCTCCACGACATTCAGCCGTGGCGTGAAAGCGAAATTCACCGCTGAACAA
AGCGAACGACTGGTGCGTATTATTCGCGTTATTGATCGGGCGGTTGAATTGTTCGAAGGCGATAAGGATGAAGCGCAAAA
ATGGCTGAATGAGCCTAACCGGGCGTTGAGCTGGAAAATGCCAGCAGAACTTATGGCATCTGAAACCGGCGCTTACGAGG
TCCTGAAACTGATCACGCGCCTGGAGAATGGGGTTTACTCTTGA