Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mosAT/- |
| Location | 3006281..3008045 | Replicon | chromosome |
| Accession | NC_012668 | ||
| Organism | Vibrio cholerae MJ-1236 | ||
Toxin (Protein)
| Gene name | mosT | Uniprot ID | A0A1L5JQJ1 |
| Locus tag | VCD_RS14000 | Protein ID | WP_000356586.1 |
| Coordinates | 3006281..3007219 (-) | Length | 313 a.a. |
Antitoxin (Protein)
| Gene name | mosA | Uniprot ID | A0A0M0HVB9 |
| Locus tag | VCD_RS14005 | Protein ID | WP_002026815.1 |
| Coordinates | 3007212..3008003 (-) | Length | 264 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VCD_RS13990 (VCD_003700) | 3001881..3002990 | - | 1110 | WP_000076135.1 | hypothetical protein | - |
| VCD_RS13995 (VCD_003701) | 3002987..3005716 | - | 2730 | WP_001901413.1 | AAA family ATPase | - |
| VCD_RS14000 (VCD_003702) | 3006281..3007219 | - | 939 | WP_000356586.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | Toxin |
| VCD_RS14005 (VCD_003703) | 3007212..3008003 | - | 792 | WP_002026815.1 | type IV toxin-antitoxin system AbiEi family antitoxin | Antitoxin |
| VCD_RS14010 (VCD_003704) | 3008223..3008609 | - | 387 | WP_000180225.1 | hypothetical protein | - |
| VCD_RS14015 (VCD_003705) | 3008606..3009178 | - | 573 | WP_001901415.1 | type IV conjugative transfer system lipoprotein TraV | - |
| VCD_RS14020 (VCD_003706) | 3009253..3010542 | - | 1290 | WP_000647307.1 | TraB/VirB10 family protein | - |
| VCD_RS14025 (VCD_003707) | 3010542..3011441 | - | 900 | WP_001901416.1 | type-F conjugative transfer system secretin TraK | - |
| VCD_RS14030 (VCD_003708) | 3011425..3012051 | - | 627 | WP_000667167.1 | hypothetical protein | - |
| VCD_RS14035 (VCD_003709) | 3012048..3012329 | - | 282 | WP_000433889.1 | type IV conjugative transfer system protein TraL | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | dfrA1 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR | - | 2916746..3071439 | 154693 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 313 a.a. Molecular weight: 35482.75 Da Isoelectric Point: 5.0041
>T6021 WP_000356586.1 NC_012668:c3007219-3006281 [Vibrio cholerae MJ-1236]
MDKDSPYYKQVSLLIRMLPVVATERVFALKGGTAINLFVRDIPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLDEKFQAEFDGMTFEKVECEDLASVRPIMLTALQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE
MDKDSPYYKQVSLLIRMLPVVATERVFALKGGTAINLFVRDIPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLDEKFQAEFDGMTFEKVECEDLASVRPIMLTALQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE
Download Length: 939 bp
>T6021 NC_012668:c3007219-3006281 [Vibrio cholerae MJ-1236]
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCAACAGAGAGGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATATTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGCGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTCGATGTGCGTATGTTACTGGGCAGTGAAGGGATTTCGAGAGAGATTTTAGTGGGTTTTTTAACCTA
TACATTAAGTCATCCCCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCTCTGGATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCTATCATGTTAACTGCACTGCAAAAA
CATTTCACAGAAAGGGATCATGCCTTCCTCATGTCATTCAAAAGAGGGCAACCTGACTGGGCATTGTTTGATTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAGTTGCAGAACATTAACAAATTGGCAAAGAATCAAGCAAAACATCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCAACAGAGAGGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATATTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGCGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTCGATGTGCGTATGTTACTGGGCAGTGAAGGGATTTCGAGAGAGATTTTAGTGGGTTTTTTAACCTA
TACATTAAGTCATCCCCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCTCTGGATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCTATCATGTTAACTGCACTGCAAAAA
CATTTCACAGAAAGGGATCATGCCTTCCTCATGTCATTCAAAAGAGGGCAACCTGACTGGGCATTGTTTGATTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAGTTGCAGAACATTAACAAATTGGCAAAGAATCAAGCAAAACATCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA
Antitoxin
Download Length: 264 a.a. Molecular weight: 30116.50 Da Isoelectric Point: 9.1901
>AT6021 WP_002026815.1 NC_012668:c3008003-3007212 [Vibrio cholerae MJ-1236]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLCSGVYYRPDAQGDIKPTWVDAIQALDVQLGVSVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLCSGVYYRPDAQGDIKPTWVDAIQALDVQLGVSVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG
Download Length: 792 bp
>AT6021 NC_012668:c3008003-3007212 [Vibrio cholerae MJ-1236]
ATGTCATCTAAGATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
TGTGAGCTACTCCCTGGCTCAAAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTATGTTCTGGCGTGTATTATCGTCCAG
ATGCGCAGGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTGGATGTGCAATTGGGCGTTTCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTGCAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAATAAGTCGTCCTTACCGAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGCGGAAACCATAAGCTTGAAG
TGAATCCAGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAACTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGACGCGATTGGGAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAGCGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCATAGATGAAACAAGAATTAAATTGGGGGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA
ATGTCATCTAAGATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
TGTGAGCTACTCCCTGGCTCAAAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTATGTTCTGGCGTGTATTATCGTCCAG
ATGCGCAGGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTGGATGTGCAATTGGGCGTTTCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTGCAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAATAAGTCGTCCTTACCGAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGCGGAAACCATAAGCTTGAAG
TGAATCCAGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAACTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGACGCGATTGGGAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAGCGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCATAGATGAAACAAGAATTAAATTGGGGGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1L5JQJ1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0M0HVB9 |
References
(1) Rachel A F Wozniak et al. (2009) A toxin-antitoxin system promotes the maintenance of an integrative conjugative element. PLoS Genetics 5(3):e1000439. [PubMed:19325886]