Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 183016..183752 Replicon plasmid unnamed1
Accession NZ_CP014121
Organism Klebsiella pneumoniae strain FDAARGOS_156

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AL516_RS01055 Protein ID WP_003026803.1
Coordinates 183270..183752 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AL516_RS01050 Protein ID WP_003026799.1
Coordinates 183016..183282 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL516_RS01000 178458..179114 + 657 WP_000113282.1 tetracycline resistance transcriptional repressor TetR(D) -
AL516_RS01005 179126..179830 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AL516_RS01015 180198..180467 + 270 WP_004152102.1 hypothetical protein -
AL516_RS01020 180455..181030 + 576 WP_004152103.1 hypothetical protein -
AL516_RS01025 181061..181555 + 495 WP_004152104.1 DNA-binding protein -
AL516_RS01030 181599..181967 + 369 WP_004152105.1 hypothetical protein -
AL516_RS01035 182001..182204 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AL516_RS01040 182253..182510 + 258 WP_004152107.1 hypothetical protein -
AL516_RS01045 182586..182840 + 255 WP_004152108.1 hypothetical protein -
AL516_RS01050 183016..183282 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AL516_RS01055 183270..183752 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AL516_RS01060 183953..185356 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AL516_RS01065 185385..186017 - 633 WP_001567369.1 hypothetical protein -
AL516_RS01070 186247..187593 + 1347 WP_020314316.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(D) - 1..200203 200203
- inside IScluster/Tn tet(D) - 166970..189121 22151


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T60197 WP_003026803.1 NZ_CP014121:183270-183752 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T60197 NZ_CP014121:183270-183752 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT60197 WP_003026799.1 NZ_CP014121:183016-183282 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT60197 NZ_CP014121:183016-183282 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References