Detailed information of TA system TA060188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1346626..1347155
• Replicon: chromosome
• Accession: NZ_CP014113
• Organism: Staphylococcus saprophyticus strain FDAARGOS_168

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AL528_RS06545
• Protein ID: WP_002482752.1
• Coordinates: 1346793..1347155 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A380HJK7
• Locus tag: AL528_RS06540
• Protein ID: WP_037538046.1
• Coordinates: 1346626..1346796 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL528_RS06515	1342357..1342836	+	480	WP_011302698.1	PH domain-containing protein	-
AL528_RS06520	1342829..1344367	+	1539	WP_047503187.1	PH domain-containing protein	-
AL528_RS06525	1344360..1344860	+	501	WP_061050524.1	PH domain-containing protein	-
AL528_RS06530	1344924..1345274	+	351	WP_002482750.1	holo-ACP synthase	-
AL528_RS06535	1345390..1346538	+	1149	WP_061050525.1	alanine racemase	-
AL528_RS06540	1346626..1346796	+	171	WP_037538046.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AL528_RS06545	1346793..1347155	+	363	WP_002482752.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AL528_RS06550	1347492..1348493	+	1002	WP_041080774.1	PP2C family protein-serine/threonine phosphatase	-
AL528_RS06555	1348572..1348898	+	327	WP_011302705.1	anti-sigma factor antagonist	-
AL528_RS06560	1348900..1349382	+	483	WP_002482755.1	anti-sigma B factor RsbW	-
AL528_RS06565	1349357..1350127	+	771	WP_002482756.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13498.70 Da
• Isoelectric Point: 10.3173

>T60188 WP_002482752.1 NZ_CP014113:1346793-1347155 [Staphylococcus saprophyticus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSDKKMKEVNNAIDISLGLHTIRSHKS

Nucleotide

• Length: 363 bp

>T60188 NZ_CP014113:1346793-1347155 [Staphylococcus saprophyticus]
ATGATGAGACGTGGTGACGTTTATTTAGCTGATTTATCACCAGTCCAGGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
AATTATACAAAACGATACTGGTAATAAATATAGTCCTACCGTAATTGTAGCCGCAATTACTGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAATTAGATAAAGATTCTGTTATATTGCTAGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACTTATCTTTCTGATAAAAAAATGAAAGAAGTTAATAATGCCATTGA
TATTAGTTTAGGATTACATACTATTAGGAGTCACAAATCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6304.97 Da
• Isoelectric Point: 4.0121

>AT60188 WP_037538046.1 NZ_CP014113:1346626-1346796 [Staphylococcus saprophyticus]
MASFNQSRIHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT60188 NZ_CP014113:1346626-1346796 [Staphylococcus saprophyticus]
ATGGCAAGTTTCAACCAAAGTAGAATTCATAGTATAGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGATTTGAA
CCTCTCCCTCGCAGCAGAAGCATTTCCGTTTGAATGTGAAGCTTGTGATTGCAACGAAGTATATATTAGCTCCAAGAATA
ATAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A380HJK7