Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4648228..4648846 Replicon chromosome
Accession NZ_CP014111
Organism Escherichia coli strain FDAARGOS_144

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AL502_RS24635 Protein ID WP_001291435.1
Coordinates 4648228..4648446 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AL502_RS24640 Protein ID WP_000344800.1
Coordinates 4648472..4648846 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL502_RS24600 4643516..4644088 + 573 WP_000779831.1 YbaY family lipoprotein -
AL502_RS24605 4644119..4644430 - 312 WP_000409908.1 MGMT family protein -
AL502_RS24615 4644809..4645162 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AL502_RS24620 4645204..4646754 - 1551 WP_022296625.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AL502_RS24625 4646918..4647388 - 471 WP_104731962.1 YlaC family protein -
AL502_RS24630 4647504..4648055 - 552 WP_000102539.1 maltose O-acetyltransferase -
AL502_RS24635 4648228..4648446 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AL502_RS24640 4648472..4648846 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AL502_RS24645 4649391..4652540 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
AL502_RS24650 4652563..4653756 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T60176 WP_001291435.1 NZ_CP014111:c4648446-4648228 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T60176 NZ_CP014111:c4648446-4648228 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT60176 WP_000344800.1 NZ_CP014111:c4648846-4648472 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT60176 NZ_CP014111:c4648846-4648472 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References