Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4171670..4172220 Replicon chromosome
Accession NZ_CP014111
Organism Escherichia coli strain FDAARGOS_144

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag AL502_RS22200 Protein ID WP_001160966.1
Coordinates 4171906..4172220 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AL502_RS22195 Protein ID WP_000125566.1
Coordinates 4171670..4171903 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL502_RS22170 4166743..4167030 + 288 WP_000203741.1 ferredoxin-like protein FixX -
AL502_RS22175 4167089..4168420 + 1332 WP_022296925.1 MFS transporter -
AL502_RS22180 4168528..4169058 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AL502_RS22185 4169051..4170913 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
AL502_RS22190 4171105..4171584 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AL502_RS22195 4171670..4171903 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AL502_RS22200 4171906..4172220 + 315 WP_001160966.1 CcdB family protein Toxin
AL502_RS22205 4172217..4173065 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AL502_RS22210 4173072..4173449 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AL502_RS22215 4173452..4174273 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AL502_RS22220 4174270..4175259 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AL502_RS22225 4175259..4176545 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T60174 WP_001160966.1 NZ_CP014111:4171906-4172220 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T60174 NZ_CP014111:4171906-4172220 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT60174 WP_000125566.1 NZ_CP014111:4171670-4171903 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT60174 NZ_CP014111:4171670-4171903 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References