Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4514555..4515209 Replicon chromosome
Accession NZ_CP014099
Organism Shigella sonnei strain FDAARGOS_90

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AL477_RS23720 Protein ID WP_000244777.1
Coordinates 4514802..4515209 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AL477_RS23715 Protein ID WP_000354046.1
Coordinates 4514555..4514821 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL477_RS23690 (4509724) 4509724..4510467 + 744 WP_000951937.1 SDR family oxidoreductase -
AL477_RS23695 (4510524) 4510524..4511957 - 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
AL477_RS23700 (4512002) 4512002..4512313 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AL477_RS23705 (4512477) 4512477..4513136 + 660 WP_000250274.1 hemolysin III family protein -
AL477_RS23710 (4513332) 4513332..4514312 - 981 WP_000886060.1 tRNA-modifying protein YgfZ -
AL477_RS23715 (4514555) 4514555..4514821 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AL477_RS23720 (4514802) 4514802..4515209 + 408 WP_000244777.1 protein YgfX Toxin
AL477_RS23725 (4515249) 4515249..4515770 - 522 WP_001055874.1 flavodoxin FldB -
AL477_RS23730 (4515882) 4515882..4516778 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AL477_RS23735 (4516803) 4516803..4517513 + 711 WP_000715206.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AL477_RS23740 (4517519) 4517519..4519252 + 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T60140 WP_000244777.1 NZ_CP014099:4514802-4515209 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T60140 NZ_CP014099:4514802-4515209 [Shigella sonnei]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT60140 WP_000354046.1 NZ_CP014099:4514555-4514821 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT60140 NZ_CP014099:4514555-4514821 [Shigella sonnei]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References