Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3148978..3149475 Replicon chromosome
Accession NZ_CP014099
Organism Shigella sonnei strain FDAARGOS_90

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AL477_RS16830 Protein ID WP_001173343.1
Coordinates 3148978..3149151 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AL477_RS16835 Protein ID WP_000398619.1
Coordinates 3149179..3149475 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL477_RS16805 (3144130) 3144130..3145286 + 1157 WP_087879933.1 IS3-like element IS600 family transposase -
AL477_RS16815 (3145332) 3145332..3145589 + 258 Protein_3132 lysine decarboxylase LdcC -
AL477_RS16820 (3145648) 3145648..3147105 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
AL477_RS16825 (3147342) 3147342..3148859 + 1518 WP_001295086.1 lysine--tRNA ligase -
AL477_RS16830 (3148978) 3148978..3149151 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AL477_RS16835 (3149179) 3149179..3149475 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AL477_RS16840 (3149702) 3149702..3149974 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AL477_RS16845 (3149986) 3149986..3150216 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
AL477_RS16850 (3150397) 3150397..3152028 + 1632 WP_001216495.1 sensor histidine kinase -
AL477_RS16855 (3152025) 3152025..3152744 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T60138 WP_001173343.1 NZ_CP014099:c3149151-3148978 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T60138 NZ_CP014099:c3149151-3148978 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT60138 WP_000398619.1 NZ_CP014099:c3149475-3149179 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT60138 NZ_CP014099:c3149475-3149179 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References