60135

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2377497..2378115 Replicon chromosome
Accession NZ_CP014099
Organism Shigella sonnei strain FDAARGOS_90

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AL477_RS13005 Protein ID WP_001291435.1
Coordinates 2377897..2378115 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H8V4S8
Locus tag AL477_RS13000 Protein ID WP_000344804.1
Coordinates 2377497..2377871 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL477_RS12990 (2372586) 2372586..2373779 + 1194 WP_011310152.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AL477_RS12995 (2373802) 2373802..2376951 + 3150 WP_001132464.1 efflux RND transporter permease AcrB -
AL477_RS13000 (2377497) 2377497..2377871 + 375 WP_000344804.1 Hha toxicity modulator TomB Antitoxin
AL477_RS13005 (2377897) 2377897..2378115 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AL477_RS13010 (2378286) 2378286..2378837 + 552 WP_000102564.1 maltose O-acetyltransferase -
AL477_RS13015 (2378953) 2378953..2379423 + 471 WP_000136192.1 YlaC family protein -
AL477_RS13020 (2379587) 2379587..2381135 + 1549 Protein_2386 cyclic-guanylate-specific phosphodiesterase PdeB -
AL477_RS13025 (2381177) 2381177..2381530 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AL477_RS13035 (2381909) 2381909..2382220 + 312 WP_000409911.1 MGMT family protein -
AL477_RS13040 (2382251) 2382251..2382823 - 573 WP_000779812.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T60135 WP_001291435.1 NZ_CP014099:2377897-2378115 [Shigella sonnei]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T60135 NZ_CP014099:2377897-2378115 [Shigella sonnei]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14615.43 Da        Isoelectric Point: 4.6232

>AT60135 WP_000344804.1 NZ_CP014099:2377497-2377871 [Shigella sonnei]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT60135 NZ_CP014099:2377497-2377871 [Shigella sonnei]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGAGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H8V4S8

References