Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 73542..74278 Replicon plasmid unnamed2
Accession NZ_CP014073
Organism Klebsiella quasipneumoniae strain FDAARGOS_93

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AL473_RS27365 Protein ID WP_003026803.1
Coordinates 73796..74278 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AL473_RS27360 Protein ID WP_003026799.1
Coordinates 73542..73808 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL473_RS27320 69386..69748 - 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -
AL473_RS27325 69800..70150 - 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AL473_RS27330 70508..70756 + 249 WP_004193994.1 hypothetical protein -
AL473_RS27335 70753..71964 + 1212 WP_032415728.1 hypothetical protein -
AL473_RS27340 72098..72589 + 492 WP_004206662.1 hypothetical protein -
AL473_RS27345 72650..72853 + 204 WP_004206661.1 hemolysin expression modulator Hha -
AL473_RS27350 72867..73097 + 231 WP_004206660.1 hypothetical protein -
AL473_RS27360 73542..73808 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AL473_RS27365 73796..74278 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AL473_RS27375 74573..75541 - 969 WP_072500845.1 IS5-like element IS903B family transposase -
AL473_RS27380 75597..75704 + 108 WP_086071112.1 transposase domain-containing protein -
AL473_RS27385 75738..76178 - 441 WP_004206983.1 VGR-related protein -
AL473_RS27390 76480..77292 - 813 WP_003032093.1 PfkB domain-containing protein -
AL473_RS27395 77305..78279 - 975 WP_003032095.1 SIS domain-containing protein -
AL473_RS27400 78284..79069 - 786 WP_003032097.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - mrkA / mrkB / mrkC / mrkF / mrkJ 1..151998 151998
- flank IS/Tn - - 74573..75541 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T60118 WP_003026803.1 NZ_CP014073:73796-74278 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T60118 NZ_CP014073:73796-74278 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT60118 WP_003026799.1 NZ_CP014073:73542-73808 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT60118 NZ_CP014073:73542-73808 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References