Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4268143..4268763 Replicon chromosome
Accession NZ_CP014070
Organism Citrobacter amalonaticus strain FDAARGOS_165

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag AL524_RS46710 Protein ID WP_001280991.1
Coordinates 4268545..4268763 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag AL524_RS46705 Protein ID WP_042324455.1
Coordinates 4268143..4268517 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL524_RS46695 4263266..4264459 + 1194 WP_043001556.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AL524_RS46700 4264482..4267631 + 3150 WP_043001557.1 multidrug efflux RND transporter permease subunit -
AL524_RS46705 4268143..4268517 + 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
AL524_RS46710 4268545..4268763 + 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
AL524_RS46715 4268940..4269491 + 552 WP_044258934.1 maltose O-acetyltransferase -
AL524_RS46720 4269605..4270075 + 471 WP_061070460.1 YlaC family protein -
AL524_RS46725 4270125..4270265 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AL524_RS46730 4270488..4272038 + 1551 WP_054176085.1 EAL domain-containing protein -
AL524_RS46735 4272078..4272431 - 354 WP_043001561.1 DUF1428 family protein -
AL524_RS46740 4272514..4273110 - 597 WP_061070461.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T60101 WP_001280991.1 NZ_CP014070:4268545-4268763 [Citrobacter amalonaticus]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T60101 NZ_CP014070:4268545-4268763 [Citrobacter amalonaticus]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCTTGCGACGCTGTCAGACAATTGACACACTTGAACGCGT
GATTGAGAAAAATAAATATGAATTATCTGACAATGAATTGGCTGTATTTTACTCAGCCGCCGATCACCGTCTTGCAGAAT
TGACGATGAATAAACTTTACGACAAGATCCCCTCCTCCGTCTGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT60101 WP_042324455.1 NZ_CP014070:4268143-4268517 [Citrobacter amalonaticus]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT60101 NZ_CP014070:4268143-4268517 [Citrobacter amalonaticus]
ATGGATGAATACTCACCCAAAAGACATGATATAGCGCAGCTTAAATTTCTTTGTGAAACGCTTTATCACGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTTCAACTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGAGCAGATTGATGAATATCTG
GACGACACATTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGCTT
GTTCCGTTGCTTTGTGAATGCCACCAGAGCTAACCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References