Detailed information of TA system TA060091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2565287..2565877
• Replicon: chromosome
• Accession: NZ_CP014070
• Organism: Citrobacter amalonaticus strain FDAARGOS_165

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AL524_RS38210
• Protein ID: WP_061069966.1
• Coordinates: 2565287..2565619 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: I6EL32
• Locus tag: AL524_RS38215
• Protein ID: WP_000288814.1
• Coordinates: 2565620..2565877 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL524_RS38190	2561521..2562783	+	1263	WP_061069963.1	integrase arm-type DNA-binding domain-containing protein	-
AL524_RS38195	2563552..2564436	-	885	WP_061069964.1	integrase domain-containing protein	-
AL524_RS50495	2565006..2565233	+	228	WP_061069965.1	hypothetical protein	-
AL524_RS38210	2565287..2565619	-	333	WP_061069966.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AL524_RS38215	2565620..2565877	-	258	WP_000288814.1	antitoxin	Antitoxin
AL524_RS38225	2566225..2566431	-	207	WP_061069967.1	helix-turn-helix domain-containing protein	-
AL524_RS38230	2566428..2566901	-	474	WP_061069968.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11844.59 Da
• Isoelectric Point: 8.0256

>T60091 WP_061069966.1 NZ_CP014070:c2565619-2565287 [Citrobacter amalonaticus]
MDRGEIWLVSLDPTSGHEQSGKRPVLIVSKASFNKLTQLPVIVPVTSGGNFAREAGFTVSLDGAETKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T60091 NZ_CP014070:c2565619-2565287 [Citrobacter amalonaticus]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCCACCAGCGGCCATGAACAAAGCGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCATTTAACAAGCTGACCCAGCTACCCGTTATTGTTCCCGTCACCAGCGGCGGAAACTTTGCTCGCG
AAGCCGGTTTTACCGTCTCACTGGACGGCGCGGAAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGCATACCCGACGCTGTTGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9280.76 Da
• Isoelectric Point: 4.5821

>AT60091 WP_000288814.1 NZ_CP014070:c2565877-2565620 [Citrobacter amalonaticus]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT60091 NZ_CP014070:c2565877-2565620 [Citrobacter amalonaticus]
ATGTATACAACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTGGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCTCAAAAACGTCCCCGCTATTCGC
TCGAAGAACTGTTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTATAG

Structures

Antitoxin

• AlphaFold DB: A0A085HLS8