Detailed information of TA system TA060089

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1328404..1329107
• Replicon: chromosome
• Accession: NZ_CP014070
• Organism: Citrobacter amalonaticus strain FDAARGOS_165

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AL524_RS31990
• Protein ID: WP_038636858.1
• Coordinates: 1328787..1329107 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AL524_RS31985
• Protein ID: WP_104010294.1
• Coordinates: 1328404..1328745 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL524_RS31955	1324744..1325178	+	435	WP_038636843.1	hypothetical protein	-
AL524_RS31960	1325198..1326001	+	804	WP_038636846.1	hypothetical protein	-
AL524_RS31965	1326218..1326694	+	477	WP_080700396.1	hypothetical protein	-
AL524_RS31970	1326766..1327587	+	822	WP_038636852.1	DUF945 domain-containing protein	-
AL524_RS31975	1327657..1328127	+	471	WP_038636854.1	DNA repair protein RadC	-
AL524_RS31980	1328136..1328357	+	222	WP_038636856.1	DUF987 family protein	-
AL524_RS31985	1328404..1328745	+	342	WP_104010294.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AL524_RS31990	1328787..1329107	+	321	WP_038636858.1	TA system toxin CbtA family protein	Toxin
AL524_RS31995	1329221..1330057	+	837	WP_038636861.1	DUF4942 domain-containing protein	-
AL524_RS32000	1330416..1331162	-	747	WP_061069582.1	epimerase	-
AL524_RS32005	1331173..1331463	-	291	WP_044264508.1	(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase	-
AL524_RS32010	1331512..1332387	-	876	WP_000774153.1	3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase	-
AL524_RS32015	1332416..1333438	-	1023	WP_000155278.1	autoinducer 2 ABC transporter substrate-binding protein LsrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1305255..1330057	24802

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (12-105)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12098.86 Da
• Isoelectric Point: 6.3209

>T60089 WP_038636858.1 NZ_CP014070:1328787-1329107 [Citrobacter amalonaticus]
MHISTVPVTVPVSSRLSPVQIWQQLLTYLLEHHYGLTLNDTPFHDDSAIQEHIEVGITLADAVNFLVERYELIRSDRKGF
TWQGQTPFLTAIDILRARRATGLMNI

Nucleotide

• Length: 321 bp

>T60089 NZ_CP014070:1328787-1329107 [Citrobacter amalonaticus]
ATGCATATTTCAACTGTACCGGTCACGGTGCCAGTTTCGTCACGCCTGTCGCCCGTGCAGATCTGGCAGCAACTATTAAC
TTATCTGCTGGAACACCATTACGGTCTAACGCTAAACGACACACCATTCCATGATGATTCCGCCATCCAGGAACATATCG
AAGTGGGAATAACACTGGCTGATGCGGTGAACTTTCTGGTGGAACGCTATGAACTGATACGCTCTGACCGCAAAGGGTTT
ACATGGCAAGGTCAGACACCATTTCTGACCGCTATCGATATTCTCAGAGCCAGGCGAGCTACCGGATTAATGAATATATA
A

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12577.05 Da
• Isoelectric Point: 5.5222

>AT60089 WP_104010294.1 NZ_CP014070:1328404-1328745 [Citrobacter amalonaticus]
ISGTSAEDTSCLQWGLKRNITPCFSARLVQEGNRLYFLADRAGFNSSFSDDEALRLDQAFPLIMKQLERMLTTGELDPRS
QHSVTLHHNGLTSEADTLGSHGYVYIAIYPSQP

Nucleotide

• Length: 342 bp

>AT60089 NZ_CP014070:1328404-1328745 [Citrobacter amalonaticus]
ATATCCGGCACATCGGCGGAGGACACATCCTGCCTTCAGTGGGGATTAAAACGTAATATCACGCCGTGCTTCAGCGCTCG
CCTGGTACAGGAAGGCAACCGCCTATACTTTCTGGCTGACCGGGCGGGGTTTAATAGCTCATTCAGTGACGATGAAGCAT
TGCGCCTAGATCAGGCGTTTCCACTAATAATGAAACAGCTAGAGCGGATGCTCACCACTGGAGAGCTTGATCCCCGCAGT
CAGCACTCCGTCACTCTGCATCACAACGGACTCACTAGTGAAGCTGACACTCTCGGCTCTCATGGCTACGTTTACATCGC
TATTTACCCCAGTCAGCCGTAA