Detailed information of TA system TA060087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 768329..768954
• Replicon: chromosome
• Accession: NZ_CP014070
• Organism: Citrobacter amalonaticus strain FDAARGOS_165

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A381GI10
• Locus tag: AL524_RS29270
• Protein ID: WP_042998865.1
• Coordinates: 768568..768954 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AL524_RS29265
• Protein ID: WP_192575752.1
• Coordinates: 768329..768568 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL524_RS29240	764292..764891	+	600	WP_061069447.1	glucose-1-phosphatase	-
AL524_RS29245	764885..765757	+	873	WP_044257165.1	virulence factor BrkB family protein	-
AL524_RS29250	765754..766191	+	438	WP_044265155.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AL524_RS29255	766236..767177	+	942	WP_042998862.1	fatty acid biosynthesis protein FabY	-
AL524_RS29260	767193..768119	-	927	WP_061069448.1	alpha/beta hydrolase	-
AL524_RS29265	768329..768568	+	240	WP_192575752.1	ribbon-helix-helix domain-containing protein	Antitoxin
AL524_RS29270	768568..768954	+	387	WP_042998865.1	type II toxin-antitoxin system VapC family toxin	Toxin
AL524_RS29275	768985..769914	-	930	WP_042998866.1	formate dehydrogenase accessory protein FdhE	-
AL524_RS29280	770059..770745	-	687	WP_061069450.1	response regulator transcription factor	-
AL524_RS29285	770750..772246	-	1497	WP_061069451.1	HAMP domain-containing histidine kinase	-
AL524_RS29290	772264..773601	-	1338	WP_086512772.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-46)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14404.65 Da
• Isoelectric Point: 9.4114

>T60087 WP_042998865.1 NZ_CP014070:768568-768954 [Citrobacter amalonaticus]
MQKGPVLFDTNILIDLFSGRQEAQQVLEAYPPQNAISLVTWMEVMVGAKKYHQEYRTRVALSAFNIIGVSQEIAERSVNL
RQEYGMKLPDAIILATAQIHRFALVTRNTRDFAGIPGVITPYQLQAGR

Nucleotide

• Length: 387 bp

>T60087 NZ_CP014070:768568-768954 [Citrobacter amalonaticus]
ATGCAAAAGGGTCCCGTGCTGTTCGATACCAATATCCTGATTGATTTATTCAGCGGGCGCCAGGAAGCACAGCAAGTACT
GGAAGCGTATCCGCCGCAGAATGCCATCAGCCTGGTTACCTGGATGGAAGTCATGGTGGGGGCAAAAAAATACCATCAGG
AATATCGTACACGTGTGGCGTTGAGTGCGTTCAACATCATCGGCGTTTCACAGGAAATTGCTGAGCGAAGCGTGAATCTC
AGGCAGGAATATGGGATGAAACTTCCGGACGCGATTATTCTGGCTACCGCGCAGATTCATCGGTTTGCGCTTGTAACGCG
CAACACCAGAGATTTCGCGGGGATACCTGGCGTCATTACGCCTTATCAGTTGCAAGCCGGACGATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9226.41 Da
• Isoelectric Point: 4.2075

>AT60087 WP_192575752.1 NZ_CP014070:768329-768568 [Citrobacter amalonaticus]
MAGMDMGRILLDLSDEMIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISNALGLWQGCEEDGVEYQKTLREEW

Nucleotide

• Length: 240 bp

>AT60087 NZ_CP014070:768329-768568 [Citrobacter amalonaticus]
ATGGCTGGAATGGACATGGGCAGAATATTACTCGACTTATCCGATGAGATGATCAAGCGCCTTGACGATCTCAAGCAGCA
GCGGAATCTGCCGCGTGCGGAGTTGTTGCGCGAAGCGGTTGAACAGTACCTTGAAAGGCAGGGACAGGCTGAAACCACGA
TTTCCAATGCGCTGGGGCTCTGGCAGGGCTGTGAGGAAGACGGCGTTGAGTATCAGAAGACGCTGCGCGAGGAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A381GI10