Detailed information of TA system TA060082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3659576..3660210
• Replicon: chromosome
• Accession: NZ_CP014067
• Organism: Enterococcus gallinarum strain FDAARGOS_163

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A120LLY6
• Locus tag: AL523_RS17455
• Protein ID: WP_008379101.1
• Coordinates: 3659576..3659926 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AL523_RS17460
• Protein ID: WP_016621415.1
• Coordinates: 3659920..3660210 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL523_RS17425	3655239..3656600	-	1362	WP_005225442.1	hypothetical protein	-
AL523_RS17430	3656949..3657872	-	924	WP_061054427.1	peptide-methionine (S)-S-oxide reductase MsrA	-
AL523_RS17440	3658187..3658732	-	546	WP_042890449.1	cysteine hydrolase	-
AL523_RS17445	3658835..3659260	+	426	WP_016613640.1	AsnC family transcriptional regulator	-
AL523_RS17450	3659342..3659560	+	219	Protein_3321	helix-turn-helix domain-containing protein	-
AL523_RS17455	3659576..3659926	-	351	WP_008379101.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AL523_RS17460	3659920..3660210	-	291	WP_016621415.1	SpoVT/AbrB-like protein	Antitoxin
AL523_RS17465	3660470..3660637	-	168	WP_016621416.1	PTS glucose transporter subunit IIA	-
AL523_RS17470	3660983..3661081	-	99	WP_074534955.1	type I toxin-antitoxin system Fst family toxin	-
AL523_RS17475	3661169..3661927	-	759	WP_061054428.1	type I methionyl aminopeptidase	-
AL523_RS17480	3661970..3662707	-	738	WP_005225435.1	tRNA pseudouridine(38-40) synthase TruA	-
AL523_RS17485	3662773..3663252	-	480	WP_008379108.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
AL523_RS17490	3663626..3664930	+	1305	WP_008379110.1	trypsin-like peptidase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (13-96)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13116.13 Da
• Isoelectric Point: 9.3469

>T60082 WP_008379101.1 NZ_CP014067:c3659926-3659576 [Enterococcus gallinarum]
MVRKPRQGDILLLDTAPRSGHEQTGKRPYIVLSHDMIANYSNVATVAPISSTSRNYPLYVSINPDYGMKTTGKVLLDQLT
TIDYEARACVFLEKAHEHLVDELLIKVRTVFQKVSR

Nucleotide

• Length: 351 bp

>T60082 NZ_CP014067:c3659926-3659576 [Enterococcus gallinarum]
ATGGTAAGAAAACCTAGACAAGGGGATATTTTGTTATTGGATACAGCTCCTCGTTCTGGACATGAACAAACGGGAAAAAG
ACCCTATATTGTTTTAAGTCATGACATGATTGCAAATTATAGTAACGTTGCCACCGTTGCTCCAATTTCTAGTACAAGTA
GAAATTATCCGTTATATGTAAGTATTAATCCAGATTATGGAATGAAGACAACAGGAAAAGTATTGTTGGATCAATTAACG
ACAATTGATTATGAAGCCAGAGCATGTGTTTTTTTGGAAAAAGCGCATGAGCACTTGGTTGATGAGTTATTGATAAAAGT
TAGAACAGTATTTCAAAAAGTAAGTAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11249.93 Da
• Isoelectric Point: 5.6091

>AT60082 WP_016621415.1 NZ_CP014067:c3660210-3659920 [Enterococcus gallinarum]
MYIQKEVSRMEMLPIKKWGNSNGLRLPKYIMEYLGIHTDDKVKIIQEEVNGHKRLIIEAANLENELTIEELFENYSAEKN
HVVIQHLGEAVGNEKW

Nucleotide

• Length: 291 bp

>AT60082 NZ_CP014067:c3660210-3659920 [Enterococcus gallinarum]
ATGTATATACAGAAAGAGGTGTCGCGCATGGAAATGCTACCTATAAAAAAATGGGGCAATAGTAACGGTCTTAGATTGCC
AAAGTACATTATGGAGTATCTTGGCATTCATACTGATGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGACATAAAC
GGCTAATCATCGAAGCAGCAAATTTAGAAAACGAGTTAACGATTGAAGAGTTATTTGAAAATTACTCTGCTGAGAAAAAT
CATGTGGTAATTCAACATTTAGGTGAAGCAGTAGGGAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A120LLY6