Detailed information of TA system TA060081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RHH
• Location: 5065..5618
• Replicon: plasmid unnamed
• Accession: NZ_CP014066
• Organism: Achromobacter xylosoxidans strain FDAARGOS_162

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1L5QZG5
• Locus tag: AL520_RS30040
• Protein ID: WP_054471487.1
• Coordinates: 5325..5618 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6N8S2B8
• Locus tag: AL520_RS30035
• Protein ID: WP_054471486.1
• Coordinates: 5065..5337 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL520_RS29995	1127..1660	+	534	WP_000381802.1	aminoglycoside nucleotidyltransferase ANT(2'')-Ia	-
AL520_RS30000	1817..2164	+	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
AL520_RS30005	2158..2997	+	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
AL520_RS30015	3125..3625	+	501	WP_000376623.1	GNAT family N-acetyltransferase	-
AL520_RS30020	3973..4737	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
AL520_RS30560	4877..4954	-	78	Protein_6	EAL domain-containing protein	-
AL520_RS30035	5065..5337	+	273	WP_054471486.1	CopG family ribbon-helix-helix protein	Antitoxin
AL520_RS30040	5325..5618	+	294	WP_054471487.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL520_RS30565	5618..5788	+	171	WP_165601277.1	hypothetical protein	-
AL520_RS30045	5785..6000	+	216	Protein_10	transcriptional regulator	-
AL520_RS30050	6055..6393	+	339	WP_052267692.1	hypothetical protein	-
AL520_RS30055	6608..6841	-	234	WP_011997484.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
AL520_RS30060	6905..7162	-	258	WP_054471488.1	hypothetical protein	-
AL520_RS30065	7165..8826	-	1662	WP_054471489.1	P-type conjugative transfer protein TrbL	-
AL520_RS30070	8823..9092	-	270	WP_054471490.1	entry exclusion lipoprotein TrbK	-
AL520_RS30075	9108..9893	-	786	WP_054471491.1	P-type conjugative transfer protein TrbJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	qacE / sul1	-	1..18415	18415
	-	flank	IS/Tn	qacE / sul1	-	1817..4737	2920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10811.34 Da
• Isoelectric Point: 5.1583

>T60081 WP_054471487.1 NZ_CP014066:5325-5618 [Achromobacter xylosoxidans]
VPQVIVTEGAAEGLERCRRFLAVKAPDAARRAGQAIERQFLLLETAPDIGRPFPEMPELRELVIAFGDSGYVALYRHEPA
DDAVYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T60081 NZ_CP014066:5325-5618 [Achromobacter xylosoxidans]
GTGCCACAAGTAATCGTCACCGAAGGCGCGGCGGAAGGCTTGGAACGATGCCGGCGGTTCCTGGCCGTCAAGGCACCGGA
CGCCGCCAGGCGTGCCGGCCAGGCCATCGAGCGGCAATTCCTGCTGCTGGAGACGGCCCCCGACATTGGCCGGCCGTTCC
CTGAAATGCCCGAGCTGCGCGAGCTGGTGATTGCCTTCGGTGATTCCGGCTATGTGGCTTTGTACCGCCACGAACCGGCC
GACGATGCGGTGTATGTTCTGGCCTTCCGGCACCAGAAAGAGGCGGGCTACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10563.86 Da
• Isoelectric Point: 8.0869

>AT60081 WP_054471486.1 NZ_CP014066:5065-5337 [Achromobacter xylosoxidans]
MATSLKIDDTLKGRVQHLASQRRRSPHWIMLEAIQQYVEREEARESFKQEALASWAAYKETGRHLTGQEVRTWLNTWGTN
DERAVPECHK

Nucleotide

• Length: 273 bp

>AT60081 NZ_CP014066:5065-5337 [Achromobacter xylosoxidans]
ATGGCAACGTCTCTCAAGATCGACGACACGCTGAAAGGTCGCGTCCAGCACCTCGCCAGCCAGCGCCGCCGCTCGCCCCA
CTGGATCATGCTCGAAGCGATCCAGCAGTACGTCGAGCGCGAAGAAGCCCGCGAGAGCTTCAAGCAGGAAGCCTTGGCGT
CCTGGGCGGCCTACAAGGAAACCGGCCGCCACCTGACCGGCCAGGAAGTTCGCACCTGGTTGAACACCTGGGGCACCAAT
GACGAAAGGGCGGTGCCTGAGTGCCACAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L5QZG5

Antitoxin

• AlphaFold DB: A0A6N8S2B8