Detailed information of TA system TA060077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 306908..307481
• Replicon: chromosome
• Accession: NZ_CP014065
• Organism: Achromobacter xylosoxidans strain FDAARGOS_162

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A5K4XXH8
• Locus tag: AL520_RS01455
• Protein ID: WP_049071622.1
• Coordinates: 306908..307285 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AL520_RS01460
• Protein ID: WP_054471260.1
• Coordinates: 307272..307481 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL520_RS01425	302743..303474	+	732	WP_054416509.1	IclR family transcriptional regulator	-
AL520_RS01430	303565..304494	+	930	WP_054416508.1	septum site-determining protein MinC	-
AL520_RS01435	304683..305498	+	816	WP_049077490.1	septum site-determining protein MinD	-
AL520_RS01440	305502..305759	+	258	WP_006388977.1	cell division topological specificity factor MinE	-
AL520_RS01445	305890..306115	-	226	Protein_278	glycine zipper 2TM domain-containing protein	-
AL520_RS01450	306307..306840	-	534	WP_049071623.1	isochorismatase family protein	-
AL520_RS01455	306908..307285	-	378	WP_049071622.1	type II toxin-antitoxin system VapC family toxin	Toxin
AL520_RS01460	307272..307481	-	210	WP_054471260.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AL520_RS01465	307606..309279	-	1674	WP_054416507.1	energy-dependent translational throttle protein EttA	-
AL520_RS01470	309430..310248	+	819	WP_054416506.1	AAC(3) family N-acetyltransferase	-
AL520_RS01475	310262..311188	-	927	WP_080977321.1	DMT family transporter	-
AL520_RS01480	311288..312076	+	789	WP_054416505.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13910.18 Da
• Isoelectric Point: 6.4885

>T60077 WP_049071622.1 NZ_CP014065:c307285-306908 [Achromobacter xylosoxidans]
MILVDTSIWIDHLSDGEPYLVRLLEEESVLMHPFIAGELALGSLGRRDVVLGAMSLLPQVVRASHDEVMHFLQSEKLFGK
GIGYVDLHLLASTRLTPGAALWTRDKRLRALANTLSLAVDPPRYH

Nucleotide

• Length: 378 bp

>T60077 NZ_CP014065:c307285-306908 [Achromobacter xylosoxidans]
ATGATCCTGGTTGACACTTCCATCTGGATAGACCATTTATCCGACGGCGAGCCATATCTGGTGCGCTTGCTGGAGGAAGA
AAGTGTATTGATGCACCCGTTCATCGCGGGTGAATTGGCGCTGGGTTCGTTGGGCCGACGTGATGTCGTATTGGGTGCGA
TGTCGCTATTGCCTCAAGTCGTGCGTGCGAGTCATGACGAAGTCATGCACTTTCTTCAAAGCGAGAAATTGTTTGGAAAG
GGCATCGGCTATGTGGATTTACACTTGTTGGCGTCCACGCGACTGACACCTGGCGCTGCGCTATGGACCCGGGACAAACG
GCTGCGGGCACTCGCCAACACCTTGAGCCTGGCGGTTGATCCGCCGAGATATCATTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7521.62 Da
• Isoelectric Point: 9.9435

>AT60077 WP_054471260.1 NZ_CP014065:c307481-307272 [Achromobacter xylosoxidans]
VRTTVTLDDDLLELARTYTGVQETSALVTRALTQLVQKEAAKRLAKLGGSSPKLKPIPRRQTETCNDPG

Nucleotide

• Length: 210 bp

>AT60077 NZ_CP014065:c307481-307272 [Achromobacter xylosoxidans]
GTGCGTACGACTGTGACGCTCGATGACGACCTGCTCGAATTGGCCCGTACCTATACCGGCGTCCAGGAGACATCGGCGCT
CGTAACGCGAGCGTTGACCCAGCTCGTGCAGAAAGAGGCCGCGAAGCGGTTGGCCAAGCTTGGTGGTAGTTCGCCCAAGT
TGAAGCCTATCCCGCGGCGGCAGACGGAAACCTGCAATGATCCTGGTTGA

Structures

Toxin

• AlphaFold DB: A0A5K4XXH8