Detailed information of TA system TA060069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1299141..1299670
• Replicon: chromosome
• Accession: NZ_CP014064
• Organism: Staphylococcus aureus strain FDAARGOS_159

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AL519_RS07155
• Protein ID: WP_000621175.1
• Coordinates: 1299141..1299503 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AL519_RS07160
• Protein ID: WP_000948331.1
• Coordinates: 1299500..1299670 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL519_RS07130	1296119..1296889	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
AL519_RS07135	1296864..1297343	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AL519_RS07140	1297345..1297671	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AL519_RS07145	1297790..1298791	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AL519_RS07155	1299141..1299503	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AL519_RS07160	1299500..1299670	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AL519_RS07165	1299755..1300903	-	1149	WP_001281152.1	alanine racemase	-
AL519_RS07170	1300969..1301328	-	360	WP_000581197.1	holo-ACP synthase	-
AL519_RS07175	1301332..1301823	-	492	WP_001205912.1	PH domain-containing protein	-
AL519_RS07180	1301810..1303393	-	1584	WP_001294626.1	PH domain-containing protein	-
AL519_RS07185	1303386..1303865	-	480	WP_001287087.1	hypothetical protein	-
AL519_RS07190	1304073..1304633	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T60069 WP_000621175.1 NZ_CP014064:c1299503-1299141 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T60069 NZ_CP014064:c1299503-1299141 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT60069 WP_000948331.1 NZ_CP014064:c1299670-1299500 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT60069 NZ_CP014064:c1299670-1299500 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1