Detailed information of TA system TA060065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1103080..1103856
• Replicon: chromosome
• Accession: NZ_CP014064
• Organism: Staphylococcus aureus strain FDAARGOS_159

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: AL519_RS05895
• Protein ID: WP_000031106.1
• Coordinates: 1103080..1103232 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: AL519_RS05900
• Protein ID: WP_001251224.1
• Coordinates: 1103257..1103856 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL519_RS05880	1099282..1100103	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
AL519_RS05885	1100556..1101941	-	1386	WP_000116228.1	class II fumarate hydratase	-
AL519_RS05890	1102137..1102532	-	396	WP_000901023.1	hypothetical protein	-
AL519_RS05895	1103080..1103232	-	153	WP_000031106.1	hypothetical protein	Toxin
AL519_RS05900	1103257..1103856	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
AL519_RS05905	1104015..1104485	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
AL519_RS05910	1104490..1105617	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
AL519_RS05915	1105768..1106490	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
AL519_RS05920	1106483..1107940	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5996.38 Da
• Isoelectric Point: 3.8962

>T60065 WP_000031106.1 NZ_CP014064:c1103232-1103080 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNFEEDSQ

Nucleotide

• Length: 153 bp

>T60065 NZ_CP014064:c1103232-1103080 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTTTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT60065 WP_001251224.1 NZ_CP014064:c1103856-1103257 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT60065 NZ_CP014064:c1103856-1103257 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: W8U4V4