Detailed information of TA system TA060062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 45272..46063
• Replicon: chromosome
• Accession: NZ_CP014064
• Organism: Staphylococcus aureus strain FDAARGOS_159

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AL519_RS00525
• Protein ID: WP_000525003.1
• Coordinates: 45272..45736 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0E7YIA0
• Locus tag: AL519_RS00530
• Protein ID: WP_000333630.1
• Coordinates: 45749..46063 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL519_RS00500	40907..41371	+	465	WP_001010508.1	SUF system NifU family Fe-S cluster assembly protein	-
AL519_RS00505	41522..42919	+	1398	WP_001074405.1	Fe-S cluster assembly protein SufB	-
AL519_RS00510	42987..44036	-	1050	WP_033859694.1	site-specific integrase	-
AL519_RS00515	44149..44328	+	180	WP_000337827.1	hypothetical protein	-
AL519_RS00520	44308..45240	-	933	WP_000392183.1	hypothetical protein	-
AL519_RS00525	45272..45736	-	465	WP_000525003.1	hypothetical protein	Toxin
AL519_RS00530	45749..46063	-	315	WP_000333630.1	helix-turn-helix domain-containing protein	Antitoxin
AL519_RS00535	46215..46451	+	237	WP_001121116.1	helix-turn-helix domain-containing protein	-
AL519_RS00540	46465..46644	+	180	WP_000438352.1	hypothetical protein	-
AL519_RS00545	46745..47227	-	483	WP_000394410.1	hypothetical protein	-
AL519_RS00550	47287..48036	+	750	WP_015978301.1	phage antirepressor KilAC domain-containing protein	-
AL519_RS00555	48052..48195	+	144	WP_000939498.1	hypothetical protein	-
AL519_RS00560	48205..48858	-	654	WP_001556705.1	hypothetical protein	-
AL519_RS00565	48929..49150	+	222	WP_000594789.1	hypothetical protein	-
AL519_RS00570	49143..49304	+	162	WP_000066017.1	DUF1270 domain-containing protein	-
AL519_RS00575	49396..49656	+	261	WP_000291090.1	DUF1108 family protein	-
AL519_RS00580	49666..49887	+	222	WP_000815403.1	DUF2483 family protein	-
AL519_RS00585	49880..50668	+	789	WP_000134097.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	41522..87408	45886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-62)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 18156.52 Da
• Isoelectric Point: 4.7927

>T60062 WP_000525003.1 NZ_CP014064:c45736-45272 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKLHHID

Nucleotide

• Length: 465 bp

>T60062 NZ_CP014064:c45736-45272 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTGATAAAGCGTGGTTTATCAGATAGAGAAAAGGCAGGGATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCTGTTCCATTACGCGAAATTGTAGAAGCTTATAATTATGGCGTACGTAACTTGTATGAATT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCGATCACATTTGAGCCGTTGAGAGTTTTTAAATTGCATCACATTGATTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.56 Da
• Isoelectric Point: 6.4736

>AT60062 WP_000333630.1 NZ_CP014064:c46063-45749 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 315 bp

>AT60062 NZ_CP014064:c46063-45749 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAACGTTATGAAAGCGGAAATATCAAAAATCTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCGTATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCATCTT
GAAGGAGAATTGACAGATGACGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E7YIA0