Detailed information of TA system TA060060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 3907079..3907632
• Replicon: chromosome
• Accession: NZ_CP014062
• Organism: Pseudomonas monteilii strain FDAARGOS_171

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q88JZ3
• Locus tag: AL532_RS18785
• Protein ID: WP_010953434.1
• Coordinates: 3907339..3907632 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2W5CWX7
• Locus tag: AL532_RS18780
• Protein ID: WP_017940300.1
• Coordinates: 3907079..3907351 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL532_RS18745	3902309..3902485	-	177	WP_182816532.1	hypothetical protein	-
AL532_RS18750	3902497..3902994	-	498	WP_104834496.1	DNA repair protein RadC	-
AL532_RS18755	3903024..3903932	-	909	WP_104834497.1	hydrolase or metal-binding protein	-
AL532_RS18760	3904024..3905028	-	1005	WP_104834498.1	YqaJ viral recombinase family protein	-
AL532_RS18765	3905108..3906076	-	969	WP_045633504.1	DUF932 domain-containing protein	-
AL532_RS18770	3906174..3906503	-	330	WP_074859151.1	hypothetical protein	-
AL532_RS18775	3906708..3906953	+	246	WP_070087259.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AL532_RS18780	3907079..3907351	+	273	WP_017940300.1	CopG family ribbon-helix-helix protein	Antitoxin
AL532_RS18785	3907339..3907632	+	294	WP_010953434.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL532_RS18790	3907626..3909035	-	1410	WP_104834499.1	site-specific integrase	-
AL532_RS18795	3909349..3910527	-	1179	Protein_3563	tyrosine-type recombinase/integrase	-
AL532_RS28300	3910822..3910998	-	177	WP_164524676.1	hypothetical protein	-
AL532_RS18800	3911434..3911718	-	285	WP_104835447.1	helix-turn-helix transcriptional regulator	-
AL532_RS18805	3911801..3912367	-	567	WP_060497295.1	hypothetical protein	-
AL532_RS18810	3912364..3912618	-	255	WP_104834500.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3902309..3915471	13162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11155.61 Da
• Isoelectric Point: 5.9081

>T60060 WP_010953434.1 NZ_CP014062:3907339-3907632 [Pseudomonas monteilii]
VPRLIVTEGAAKGLERCRRFLSDKDPQVARRAAQAIERQFARLEESPEVGRPFPDLPELRELIIEFGDSGYVALYRYERA
DDTAYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T60060 NZ_CP014062:3907339-3907632 [Pseudomonas monteilii]
GTGCCACGACTGATCGTCACTGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GGTCGCACGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCATTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATCGAGTTTGGTGACTCAGGTTACGTCGCGCTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10327.45 Da
• Isoelectric Point: 5.5751

>AT60060 WP_017940300.1 NZ_CP014062:3907079-3907351 [Pseudomonas monteilii]
MATSIKIDDDLKGRIQHLAGLRQRSSHWIMREAITQYVEREEARESFKQEALASWAAYQETGQHLTGQEARTWLGSWGTD
AEAELPKCHD

Nucleotide

• Length: 273 bp

>AT60060 NZ_CP014062:3907079-3907351 [Pseudomonas monteilii]
ATGGCCACTTCCATCAAAATTGACGACGATCTGAAAGGTCGCATTCAGCATTTGGCTGGTTTGCGCCAGCGCTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCATACCAGGAAACCGGCCAGCACTTGACCGGCCAGGAGGCTCGCACCTGGCTTGGTAGTTGGGGTACAGAT
GCTGAGGCAGAGTTACCCAAGTGCCACGACTGA

Structures

Toxin

• AlphaFold DB: A0A2W5CNE8

Antitoxin

• AlphaFold DB: A0A2W5CWX7