Detailed information of TA system TA060059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2489900..2490486
• Replicon: chromosome
• Accession: NZ_CP014062
• Organism: Pseudomonas monteilii strain FDAARGOS_171

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: AL532_RS11920
• Protein ID: WP_082426425.1
• Coordinates: 2489900..2490193 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A923H277
• Locus tag: AL532_RS11925
• Protein ID: WP_060499950.1
• Coordinates: 2490190..2490486 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL532_RS11895	2485549..2486403	+	855	WP_060499955.1	putative selenate ABC transporter substrate-binding protein	-
AL532_RS11900	2486400..2487209	+	810	WP_104833972.1	ATP-binding cassette domain-containing protein	-
AL532_RS11905	2487203..2488030	+	828	WP_104833973.1	ABC transporter permease	-
AL532_RS11910	2488027..2488794	+	768	WP_060499952.1	phosphonate ABC transporter, permease protein PhnE	-
AL532_RS11915	2488791..2489678	-	888	WP_104833974.1	effector protein	-
AL532_RS11920	2489900..2490193	+	294	WP_082426425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AL532_RS11925	2490190..2490486	+	297	WP_060499950.1	putative addiction module antidote protein	Antitoxin
AL532_RS11930	2490488..2490976	-	489	WP_104833975.1	GNAT family N-acetyltransferase	-
AL532_RS11935	2491128..2491775	-	648	WP_060499948.1	hypothetical protein	-
AL532_RS11940	2492071..2492577	+	507	WP_060499947.1	sigma-70 family RNA polymerase sigma factor	-
AL532_RS11945	2492577..2493518	+	942	WP_104833976.1	FecR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2488791..2531781	42990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10847.80 Da
• Isoelectric Point: 10.5184

>T60059 WP_082426425.1 NZ_CP014062:2489900-2490193 [Pseudomonas monteilii]
MYLVEQTESFAVWLSSLRDLKAKLAIGRRLERVAIGNLGDFKWLGGGLGELRIDVGAGYRVYFTQKGHRLVLLLVGGDKS
TQVADILKSRKLLKEMI

Nucleotide

• Length: 294 bp

>T60059 NZ_CP014062:2489900-2490193 [Pseudomonas monteilii]
ATGTATCTTGTTGAACAGACGGAGAGCTTTGCAGTTTGGCTATCCAGTTTGAGGGATTTGAAAGCCAAGCTTGCGATAGG
TCGCAGGCTGGAGCGAGTTGCGATAGGCAACCTTGGTGACTTCAAGTGGCTTGGAGGCGGTCTAGGTGAGCTTCGCATCG
ACGTAGGTGCAGGCTATCGCGTCTATTTCACGCAAAAGGGGCACAGATTGGTTCTGCTGCTGGTTGGCGGCGACAAGTCC
ACTCAAGTCGCCGATATTCTCAAATCAAGAAAGCTGCTGAAGGAGATGATATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10587.22 Da
• Isoelectric Point: 6.2205

>AT60059 WP_060499950.1 NZ_CP014062:2490190-2490486 [Pseudomonas monteilii]
MSEKLIPFDMAALLSSDEAISEYLSQVLSDGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLK
VIRALGVDLLVQPHAVAR

Nucleotide

• Length: 297 bp

>AT60059 NZ_CP014062:2490190-2490486 [Pseudomonas monteilii]
ATGAGCGAAAAACTGATTCCGTTTGATATGGCAGCTTTGTTGAGTAGCGATGAGGCCATCAGTGAGTACCTTAGCCAGGT
GCTATCAGATGGCGATACCGAAGAGATCATTCGAGCGCTCGGCCATATCGCGCGTGCACGAGGCATGACACACATAGCCA
CGGAGAGTGGTTTGGGCAGAGAAAGCCTCTACAAGGCGCTCAGCCCAGGCGCAAAGCCTCGTTTTGACACTGTGTTGAAG
GTCATTCGAGCCTTGGGGGTAGACCTTCTCGTGCAGCCCCATGCGGTAGCCCGTTGA