Detailed information of TA system TA060036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 60630..61989
• Replicon: plasmid unnamed1
• Accession: NZ_CP014052
• Organism: Vibrio alginolyticus strain FDAARGOS_108

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: AL539_RS00330
• Protein ID: WP_104975141.1
• Coordinates: 60630..61583 (-)
• Length: 318 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A161BXZ5
• Locus tag: AL539_RS00335
• Protein ID: WP_005391098.1
• Coordinates: 61585..61989 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL539_RS00315	56930..57787	-	858	WP_104975138.1	hypothetical protein	-
AL539_RS00320	57867..59102	-	1236	WP_104975139.1	restriction endonuclease subunit S	-
AL539_RS00325	59092..60609	-	1518	WP_104975140.1	type I restriction-modification system subunit M	-
AL539_RS00330	60630..61583	-	954	WP_104975141.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
AL539_RS00335	61585..61989	-	405	WP_005391098.1	hypothetical protein	Antitoxin
AL539_RS00340	62178..62771	-	594	WP_005391099.1	tyrosine-type recombinase/integrase	-
AL539_RS00345	63266..63568	+	303	WP_014386665.1	hypothetical protein	-
AL539_RS00350	63852..64628	-	777	WP_192945246.1	sel1 repeat family protein	-
AL539_RS00355	64851..65573	-	723	WP_041154780.1	NYN domain-containing protein	-
AL539_RS00360	65636..66355	-	720	WP_032073007.1	hypothetical protein	-
AL539_RS00365	66501..66761	-	261	WP_046224235.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..217123	217123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-304)

Antitoxin

• Protein of unknown function DUF6088 (7-129)

Sequences

Toxin

Protein

• Length: 318 a.a.
• Molecular weight: 36339.96 Da
• Isoelectric Point: 5.4975

>T60036 WP_104975141.1 NZ_CP014052:c61583-60630 [Vibrio alginolyticus]
MEKLKQQFLEVSDALELGNPAIIEKDYWVVALLSELAEVKPDSHQMVFSGGTALAKSNVKILRMSEDVDIKLIPKQAFDE
LSRAKKKAARKACINGIEQAITRSERFTVEARSVSDEYRYVELELRYPQQFSQAPCLRPIIKLELMETIPLFDAEPRSIQ
SLVAELYKQPPEVKAFDCVSIHATLVEKIISMLRRTMSVKRDAQRKDDATLVRHIYDVYCILKTEEQTEHLDLTALTALF
KTVLEEDIVRFGNQHAEFVATPKQELQLGLQELEENPVFRHRFQDFVTPMVFNTEPHDFDTCFASFKCIAASLIKTI

Nucleotide

• Length: 954 bp

>T60036 NZ_CP014052:c61583-60630 [Vibrio alginolyticus]
ATGGAAAAACTAAAGCAACAGTTTCTTGAGGTTTCCGATGCACTTGAGCTCGGAAACCCTGCCATTATTGAAAAAGATTA
CTGGGTAGTTGCCTTGCTTTCTGAGCTAGCAGAAGTGAAGCCTGACTCTCACCAAATGGTCTTTTCTGGCGGTACAGCTC
TGGCAAAGTCAAACGTTAAAATTCTGAGAATGTCAGAAGACGTAGACATTAAATTGATCCCTAAGCAAGCGTTTGATGAA
CTGAGCCGCGCAAAAAAGAAAGCAGCTCGTAAGGCATGCATCAACGGTATTGAACAGGCGATCACTCGGTCAGAGCGATT
TACGGTTGAAGCGCGTAGTGTAAGTGATGAATATCGCTATGTTGAGTTGGAACTCCGATACCCGCAACAGTTTAGCCAAG
CGCCATGTCTTCGCCCCATTATCAAACTGGAGTTAATGGAGACAATCCCACTGTTTGATGCAGAGCCTCGTAGCATTCAA
TCACTGGTCGCAGAGCTGTATAAACAACCACCAGAAGTGAAGGCGTTTGATTGCGTTTCTATCCATGCCACGTTGGTTGA
AAAAATCATATCAATGTTGCGCCGCACAATGTCAGTCAAGCGCGATGCACAGCGTAAGGATGATGCCACCCTTGTGCGCC
ATATCTATGATGTTTACTGCATTCTAAAGACCGAAGAACAGACAGAACACCTCGACCTGACAGCACTTACAGCGTTATTT
AAAACTGTGTTGGAAGAAGATATTGTGCGTTTTGGTAATCAGCATGCAGAGTTTGTTGCCACTCCAAAACAAGAGCTACA
ACTCGGATTGCAAGAACTTGAAGAAAACCCTGTTTTCCGCCATCGCTTTCAAGACTTTGTCACTCCTATGGTGTTTAACA
CCGAACCTCATGATTTTGATACCTGCTTTGCCTCCTTTAAATGCATTGCAGCGTCGCTAATTAAAACAATTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15209.56 Da
• Isoelectric Point: 10.6291

>AT60036 WP_005391098.1 NZ_CP014052:c61989-61585 [Vibrio alginolyticus]
MTAVDRIYQKIKRSRRYVFERKDFDGFASYDQIGRALKQLVKKGELIKLGYGLYTKARINSLTGKPMPTNPGGSDALMRE
ILKMKGVDFEMDNLSLQSLSGESTQIPSSVQYSWNPKQFNRKLVVGNRVLNSPS

Nucleotide

• Length: 405 bp

>AT60036 NZ_CP014052:c61989-61585 [Vibrio alginolyticus]
ATGACAGCCGTTGACCGTATTTATCAAAAGATAAAGCGTTCAAGACGTTATGTGTTTGAGCGCAAAGACTTTGATGGCTT
TGCTAGCTACGATCAAATTGGTCGTGCGCTAAAGCAACTAGTCAAAAAAGGTGAGTTAATCAAGCTTGGCTATGGTCTAT
ACACCAAGGCAAGGATTAACAGCTTAACGGGCAAACCCATGCCTACGAACCCTGGGGGGAGCGATGCCCTGATGCGCGAG
ATATTAAAGATGAAAGGCGTCGATTTTGAGATGGACAACCTATCTTTGCAAAGCCTATCAGGTGAAAGCACCCAAATCCC
ATCATCCGTTCAATACTCATGGAATCCCAAACAATTTAATCGCAAATTGGTTGTTGGTAACCGAGTGTTGAATTCACCGA
GTTAA

Structures

Antitoxin

• AlphaFold DB: A0A161BXZ5