Detailed information of TA system TA060032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1409220..1409718
• Replicon: chromosome
• Accession: NZ_CP014049
• Organism: Vibrio vulnificus strain FDAARGOS_119

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A8H9N5A4
• Locus tag: AL549_RS15115
• Protein ID: WP_058666912.1
• Coordinates: 1409452..1409718 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8H9N567
• Locus tag: AL549_RS15110
• Protein ID: WP_033928110.1
• Coordinates: 1409220..1409465 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AL549_RS15045	1404393..1404521	-	129	WP_081090719.1	DUF3265 domain-containing protein	-
AL549_RS15050	1404518..1404904	-	387	WP_045597227.1	tautomerase family protein	-
AL549_RS15055	1405043..1405480	-	438	WP_061058665.1	GNAT family N-acetyltransferase	-
AL549_RS24040	1405496..1405567	-	72	WP_192945298.1	hypothetical protein	-
AL549_RS24045	1405663..1405968	-	306	WP_061058666.1	hypothetical protein	-
AL549_RS24050	1405978..1406070	-	93	WP_192945291.1	DUF3265 domain-containing protein	-
AL549_RS15070	1406102..1406575	-	474	WP_058666908.1	hypothetical protein	-
AL549_RS15075	1406743..1407231	-	489	WP_031781292.1	hypothetical protein	-
AL549_RS15080	1407259..1407399	-	141	WP_081090720.1	DUF3265 domain-containing protein	-
AL549_RS15085	1407377..1407661	-	285	WP_061058667.1	nucleotide pyrophosphohydrolase	-
AL549_RS15090	1407677..1407832	-	156	WP_081090648.1	DUF3265 domain-containing protein	-
AL549_RS15095	1407874..1408119	+	246	WP_058666907.1	type II toxin-antitoxin system CcdA family antitoxin	-
AL549_RS15100	1408119..1408436	+	318	WP_017420671.1	CcdB family protein	-
AL549_RS15105	1408562..1409077	-	516	WP_058666909.1	GNAT family N-acetyltransferase	-
AL549_RS15110	1409220..1409465	-	246	WP_033928110.1	hypothetical protein	Antitoxin
AL549_RS15115	1409452..1409718	-	267	WP_058666912.1	BrnT family toxin	Toxin
AL549_RS24055	1409774..1409845	-	72	WP_114983889.1	hypothetical protein	-
AL549_RS15140	1411289..1411447	-	159	WP_081090649.1	DUF3265 domain-containing protein	-
AL549_RS15145	1411762..1411890	-	129	WP_081090721.1	DUF3265 domain-containing protein	-
AL549_RS15155	1412240..1412380	-	141	WP_081090650.1	DUF3265 domain-containing protein	-
AL549_RS15160	1412364..1412672	-	309	WP_017420768.1	hypothetical protein	-
AL549_RS15165	1412710..1412832	-	123	WP_103156313.1	DUF3265 domain-containing protein	-
AL549_RS15170	1412829..1413083	-	255	WP_045612901.1	hypothetical protein	-
AL549_RS15175	1413098..1413253	-	156	WP_104408408.1	DUF3265 domain-containing protein	-
AL549_RS15180	1413295..1413540	+	246	WP_017420672.1	type II toxin-antitoxin system CcdA family antitoxin	-
AL549_RS15185	1413540..1413857	+	318	WP_017420671.1	CcdB family protein	-
AL549_RS15190	1413991..1414359	-	369	WP_026050325.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1220087..1425467	205380
	-	inside	Integron	-	-	1224520..1425467	200947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10083.33 Da
• Isoelectric Point: 7.2017

>T60032 WP_058666912.1 NZ_CP014049:c1409718-1409452 [Vibrio vulnificus]
MIKFEFDENKSRSNLEKHGIDFNTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISIRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T60032 NZ_CP014049:c1409718-1409452 [Vibrio vulnificus]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTAATACAGCTCAAGG
GTTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAATTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9547.87 Da
• Isoelectric Point: 6.8810

>AT60032 WP_033928110.1 NZ_CP014049:c1409465-1409220 [Vibrio vulnificus]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLERVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT60032 NZ_CP014049:c1409465-1409220 [Vibrio vulnificus]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTGGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGTTGGCAGAAAGGTTAGAGCGCGTGTCTCATCATTCAAGTGTG
AGATAA